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Human WNT1 ELISA Kit

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  • Alternative name

    Human proto-oncogene Int-1 homolog ELISA Kit;Human INT1 ELISA Kit;Human BMND16 ELISA Kit;Human OI15 ELISA Kit;Human Wnt family member 1 ELISA Kit;Human proto-oncogene Wnt-1 ELISA Kit;Human wingless-type MMTV integration site family member 1 ELISA Kit;Human wingless-type MMTV integration site family, member 1 (oncogene INT1) ELISA Kit;
  • Catalog
    E032084
  • species
    Human
  • GeneWNT1
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman WNT1 ELISA Kit allows for the in vitro quantitative determination of WNT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human WNT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human WNT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human WNT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human WNT1 can be calculated.
  • Human Proto-oncogene Wnt-1 Protein information
  • Uniprot ID WNT1_HUMAN
  • Uniprot AC P04628; Q5U0N2;
  • UniGene Hs.248164;
  • GeneID 7471
  • KEGG hsa:7471;
  • Human Proto-oncogene Wnt-1 Protein SEQUENCE

  • SEQUENCE 370 AA; 40982 MW; F7E8111DA12E173F CRC64;

    MGLWALLPGW VSATLLLALA ALPAALAANS SGRWWGIVNV ASSTNLLTDS

    KSLQLVLEPS LQLLSRKQRR LIRQNPGILH SVSGGLQSAV RECKWQFRNR

    RWNCPTAPGP HLFGKIVNRG CRETAFIFAI TSAGVTHSVA RSCSEGSIES

    CTCDYRRRGP GGPDWHWGGC SDNIDFGRLF GREFVDSGEK GRDLRFLMNL

    HNNEAGRTTV FSEMRQECKC HGMSGSCTVR TCWMRLPTLR AVGDVLRDRF

    DGASRVLYGN RGSNRASRAE LLRLEPEDPA HKPPSPHDLV YFEKSPNFCT

    YSGRLGTAGT AGRACNSSSP ALDGCELLCC GRGHRTRTQR VTERCNCTFH

    WCCHVSCRNC THTRVLHECL

  • UCSC uc001rsu.4; human.;



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