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Human PCDHB15 ELISA Kit

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  • Alternative name

    Human PCDH-beta-15 ELISA Kit;Human PCDH-BETA15 ELISA Kit;Human protocadherin beta 15 ELISA Kit;Human protocadherin beta-15 ELISA Kit;
  • Catalog
    E032155
  • species
    Human
  • GenePCDHB15
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Protocadherin Beta 15 (PCDHb15). No significant cross-reactivity or interference between Protocadherin Beta 15 (PCDHb15) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.064ng/mL.
  • Intended UseHuman PCDHB15 ELISA Kit allows for the in vitro quantitative determination of PCDHB15 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protocadherin Beta 15 (PCDHb15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protocadherin Beta 15 (PCDHb15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protocadherin Beta 15 (PCDHb15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protocadherin Beta 15 (PCDHb15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protocadherin beta-15 Protein information
  • Uniprot ID PCDBF_HUMAN
  • Uniprot AC Q9Y5E8; Q8IUX5;
  • UniGene Hs.130757;
  • GeneID 56121
  • KEGG hsa:56121;
  • Human Protocadherin beta-15 Protein SEQUENCE

  • SEQUENCE 787 AA; 86329 MW; 8DE8D3A07B0B6A56 CRC64;

    MEPAGERFPE QRQVLILLLL LEVTLAGWEP RRYSVMEETE RGSFVANLAN

    DLGLGVGELA ERGARVVSED NEQGLQLDLQ TGQLILNEKL DREKLCGPTE

    PCIMHFQVLL KKPLEVFRAE LLVTDINDHS PEFPEREMTL KIPETSSLGT

    VFPLKKARDL DVGSNNVQNY NISPNSHFHV STRTRGDGRK YPELVLDTEL

    DREEQAELRL TLTAVDGGSP PRSGTVQILI LVLDANDNAP EFVQALYEVQ

    VPENSPVGSL VVKVSARDLD TGTNGEISYS LYYSSQEIDK PFELSSLSGE

    IRLIKKLDFE TMSSYDLDIE ASDGGGLSGK CSVSVKVLDV NDNFPELSIS

    SLTSPIPENS PETEVALFRI RDRDSGENGK MICSIQDDVP FKLKPSVENF

    YRLVTEGALD RETRAEYNIT ITITDLGTPR LKTEQSITVL VSDVNDNAPA

    FTQTSYTLFV RENNSPALHI GSVSATDRDS GTNAQVTYSL LPPRDPHLPL

    TSLVSINTDN GHLFALQSLD YEALQAFEFR VGATDRGFPA LSSEALVRVL

    VLDANDNSPF VLYPLQNGSA PCTELVPRAA EPGYLVTKVV AVDGDSGQNA

    WLSYQLLKAT EPGLFGVWAH NGEVRTARLL SERDVAKHRL VVLVKDNGEP

    PRSATATLQV LLVDGFSQPY LPLPEAAPAQ AQADSLTVYL VVALASVSSL

    FLFSVFLFVA VRLCRRSRAA SVGRCSVPEG PFPGHLVDVS GTGTLSQSYQ

    YEVCLTGGSE SNDFKFLKPI FPNIVSQDSR RKSEFLE

  • UCSC uc003lje.5; human.;



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