Human PRUNE ELISA Kit

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  • Alternative name

    Human hPrune ELISA Kit;Human Drosophila-related expressed sequence 17 ELISA Kit;Human DRES-17 ELISA Kit;Human DRES17 ELISA Kit;Human HTCD37 ELISA Kit;Human H-PRUNE ELISA Kit;Human PRUNE ELISA Kit;Human prune exopolyphosphatase 1 ELISA Kit;Human protein prune homolog ELISA Kit;

  • Catalog
    E032225
  • species
    Human
  • GenePRUNE
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human PRUNE. No significant cross-reactivity or interference between human PRUNE and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human PRUNE is typically less than 11.75 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
  • Intended UseHuman PRUNE ELISA Kit allows for the in vitro quantitative determination of PRUNE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRUNE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRUNE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRUNE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRUNE bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Protein prune homolog Protein information
  • Uniprot ID PRUNE_HUMAN
  • Uniprot AC Q9C071; Q9C072; Q9UIV0;
  • UniGene Hs.78524;
  • GeneID 58497
  • KEGG hsa:58497;
  • Human Protein prune homolog Protein SEQUENCE
  • SEQUENCE 453 AA; 50200 MW; 34BE1909AB89DBD5 CRC64;

    MEDYLQGCRA ALQESRPLHV VLGNEACDLD STVSALALAF YLAKTTEAEE

    VFVPVLNIKR SELPLRGDIV FFLQKVHIPE SILIFRDEID LHALYQAGQL

    TLILVDHHIL SKSDTALEEA VAEVLDHRPI EPKHCPPCHV SVELVGSCAT

    LVTERILQGA PEILDRQTAA LLHGTIILDC VNMDLKIGKA TPKDSKYVEK

    LEALFPDLPK RNDIFDSLQK AKFDVSGLTT EQMLRKDQKT IYRQGVKVAI

    SAIYMDLEAF LQRSNLLADL HAFCQAHSYD VLVAMTIFFN THNEPVRQLA

    IFCPHVALQT TICEVLERSH SPPLKLTPAS STHPNLHAYL QGNTQVSRKK

    LLPLLQEALS AYFDSMKIPS GQPETADVSR EQVDKELDRA SNSLISGLSQ

    DEEDPPLPPT PMNSLVDECP LDQGLPKLSA EAVFEKCSQI SLSQSTTASL

    SKK

  • UCSC uc001ewh.2; human. [Q86TP1-1];



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