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Human P2RY1 ELISA Kit

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  • Alternative name

    Human P2Y1 ELISA Kit;Human ATP receptor ELISA Kit;Human Purinergic receptor ELISA Kit;Human purinergic receptor P2Y1 ELISA Kit;Human P2Y purinoceptor 1 ELISA Kit;Human P2 purinoceptor subtype Y1 ELISA Kit;Human platelet ADP receptor ELISA Kit;Human purinergic receptor P2Y, G-protein coupled, 1 ELISA Kit;
  • Catalog
    E032331
  • species
    Human
  • GeneP2RY1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Purinergic Receptor P2Y, G Protein Coupled 1 (P2RY1). No significant cross-reactivity or interference between Purinergic Receptor P2Y, G Protein Coupled 1 (P2RY1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.054ng/mL.
  • Intended UseHuman P2RY1 ELISA Kit allows for the in vitro quantitative determination of P2RY1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Purinergic Receptor P2Y, G Protein Coupled 1 (P2RY1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Purinergic Receptor P2Y, G Protein Coupled 1 (P2RY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Purinergic Receptor P2Y, G Protein Coupled 1 (P2RY1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Purinergic Receptor P2Y, G Protein Coupled 1 (P2RY1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human P2Y purinoceptor 1 Protein information
  • Uniprot ID P2RY1_HUMAN
  • Uniprot AC P47900;
  • UniGene Hs.654526; Hs.79881;
  • GeneID 5028
  • KEGG hsa:5028;
  • Human P2Y purinoceptor 1 Protein SEQUENCE

  • SEQUENCE 373 AA; 42072 MW; 4DC7C668B4145392 CRC64;

    MTEVLWPAVP NGTDAAFLAG PGSSWGNSTV ASTAAVSSSF KCALTKTGFQ

    FYYLPAVYIL VFIIGFLGNS VAIWMFVFHM KPWSGISVYM FNLALADFLY

    VLTLPALIFY YFNKTDWIFG DAMCKLQRFI FHVNLYGSIL FLTCISAHRY

    SGVVYPLKSL GRLKKKNAIC ISVLVWLIVV VAISPILFYS GTGVRKNKTI

    TCYDTTSDEY LRSYFIYSMC TTVAMFCVPL VLILGCYGLI VRALIYKDLD

    NSPLRRKSIY LVIIVLTVFA VSYIPFHVMK TMNLRARLDF QTPAMCAFND

    RVYATYQVTR GLASLNSCVD PILYFLAGDT FRRRLSRATR KASRRSEANL

    QSKSEDMTLN ILPEFKQNGD TSL

  • UCSC uc003ezq.4; human.;



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