Human P2RY12 ELISA Kit

Alternative name
Human P2Y12 ELISA Kit;Human ADP-glucose receptor ELISA Kit;Human ADPG-R ELISA Kit;Human P2T(AC) ELISA Kit;Human P2Y(AC) ELISA Kit;Human P2Y(cyc) ELISA Kit;Human P2Y12 platelet ADP receptor ELISA Kit;Human P2Y(ADP) ELISA Kit;Human SP1999 ELISA Kit;Human HORK3 ELISA Kit;Human BDPLT8 ELISA Kit;Human P2Y(12)R ELISA Kit;Human purinergic receptor P2Y12 ELISA Kit;Human P2Y purinoceptor 12 ELISA Kit;Human G-protein coupled receptor SP1999 ELISA Kit;Human Gi-coupled ADP receptor HORK3 ELISA Kit;Human purinergic receptor P2RY12 ELISA Kit;Human purinergic receptor P2Y, G-protein coupled, 12 ELISA Kit;Human putative G-protein coupled receptor ELISA Kit;
Catalog
E032332
species
Human
GeneP2RY12
SpecificityThis assay has high sensitivity and excellent specificity for detection of Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12). No significant cross-reactivity or interference between Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.061ng/mL.
Intended UseHuman P2RY12 ELISA Kit allows for the in vitro quantitative determination of P2RY12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human P2Y purinoceptor 12 Protein information
Uniprot ID P2Y12_HUMAN
Uniprot AC Q9H244; D3DNJ5; Q546J7;
UniGene Hs.591281; Hs.665544;
GeneID 64805
KEGG hsa:64805;
Human P2Y purinoceptor 12 Protein SEQUENCE
SEQUENCE 342 AA; 39439 MW; 8553D2746C89176D CRC64;
MQAVDNLTSA PGNTSLCTRD YKITQVLFPL LYTVLFFVGL ITNGLAMRIF
FQIRSKSNFI IFLKNTVISD LLMILTFPFK ILSDAKLGTG PLRTFVCQVT
SVIFYFTMYI SISFLGLITI DRYQKTTRPF KTSNPKNLLG AKILSVVIWA
FMFLLSLPNM ILTNRQPRDK NVKKCSFLKS EFGLVWHEIV NYICQVIFWI
NFLIVIVCYT LITKELYRSY VRTRGVGKVP RKKVNVKVFI IIAVFFICFV
PFHFARIPYT LSQTRDVFDC TAENTLFYVK ESTLWLTSLN ACLDPFIYFF
LCKSFRNSLI SMLKCPNSAT SLSQDNRKKE QDGGDPNEET PM
UCSC uc003eyx.3; human.;

Category

Human P2RY12 ELISA Kit