Human MUM1 ELISA Kit

Alternative name
Human mutated melanoma-associated antigen 1 ELISA Kit;Human MUM-1 ELISA Kit;Human protein expandere ELISA Kit;Human EXPAND1 ELISA Kit;Human HSPC211 ELISA Kit;Human melanoma associated antigen (mutated) 1 ELISA Kit;Human PWWP domain-containing protein MUM1 ELISA Kit;Human melanoma ubiquitous mutated protein ELISA Kit;
Catalog
E032512
species
Human
GeneMUM1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<46.9pg/ml
Intended UseHuman MUM1 ELISA Kit allows for the in vitro quantitative determination of MUM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MUM1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MUM1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MUM1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MUM1 can be calculated.
Human PWWP domain-containing protein MUM1 Protein information
Uniprot ID MUM1_HUMAN
Uniprot AC Q96A67;
UniGene Hs.515016;
GeneID 84939
KEGG hsa:84939;
Human PWWP domain-containing protein MUM1 Protein SEQUENCE
SEQUENCE 710 AA; 78636 MW; 96D8A77FE814F6F8 CRC64;
MADAKYVLCR WEKRLWPAKV LARTATSTKN KRRKEYFLAV QILSLEEKIK
VKSTEVEILE KSQIEAIASS LASQNEVPAA PLEELAYRRS LRVALDVLSE
GSIWSQESSA GTGRADRSLR GKPMEHVSSP CDSNSSSLPR GDVLGSSRPH
RRRPCVQQSL SSSFTCEKDP ECKVDHKKGL RKSENPRGPL VLPAGGGAQD
ESGSRIHHKN WTLASKRGGN SAQKASLCLN GSSLSEDDTE RDMGSKGGSW
AAPSLPSGVR EDDPCANAEG HDPGLPLGSL TAPPAPEPSA CSEPGECPAK
KRPRLDGSQR PPAVQLEPMA AGAAPSPGPG PGPRESVTPR STARLGPPPS
HASADATRCL PCPDSQKLEK ECQSSEESMG SNSMRSILEE DEEDEEPPRV
LLYHEPRSFE VGMLVWHKHK KYPFWPAVVK SVRQRDKKAS VLYIEGHMNP
KMKGFTVSLK SLKHFDCKEK QTLLNQARED FNQDIGWCVS LITDYRVRLG
CGSFAGSFLE YYAADISYPV RKSIQQDVLG TKLPQLSKGS PEEPVVGCPL
GQRQPCRKML PDRSRAARDR ANQKLVEYIV KAKGAESHLR AILKSRKPSR
WLQTFLSSSQ YVTCVETYLE DEGQLDLVVK YLQGVYQEVG AKVLQRTNGD
RIRFILDVLL PEAIICAISA VDEVDYKTAE EKYIKGPSLS YREKEIFDNQ
LLEERNRRRR
UCSC uc060qzk.1; human. [Q2TAK8-1];

Category

Human MUM1 ELISA Kit