Human PYGB ELISA Kit

Alternative name
Human GPBB ELISA Kit;Human glycogen phosphorylase B ELISA Kit;Human glycogen phosphorylase, brain form ELISA Kit;Human phosphorylase, glycogen; brain ELISA Kit;
Catalog
E032518
species
Human
GenePYGB
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human PYGB. No significant cross-reactivity or interference between Human PYGB and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PYGB and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman PYGB ELISA Kit allows for the in vitro quantitative determination of PYGB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PYGB in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PYGB. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PYGB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PYGB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PYGB. You can calculate the concentration of Human PYGB in the samples by comparing the OD of the samples to the standard curve.
Human Glycogen phosphorylase, brain form Protein information
Uniprot ID PYGB_HUMAN
Uniprot AC P11216; Q96AK1; Q9NPX8;
UniGene Hs.368157;
GeneID 5834
KEGG hsa:5834;
Human Glycogen phosphorylase, brain form Protein SEQUENCE
SEQUENCE 843 AA; 96696 MW; 810BFAD3002CACB0 CRC64;
MAKPLTDSEK RKQISVRGLA GLGDVAEVRK SFNRHLHFTL VKDRNVATPR
DYFFALAHTV RDHLVGRWIR TQQHYYERDP KRIYYLSLEF YMGRTLQNTM
VNLGLQNACD EAIYQLGLDL EELEEIEEDA GLGNGGLGRL AACFLDSMAT
LGLAAYGYGI RYEFGIFNQK IVNGWQVEEA DDWLRYGNPW EKARPEYMLP
VHFYGRVEHT PDGVKWLDTQ VVLAMPYDTP VPGYKNNTVN TMRLWSAKAP
NDFKLQDFNV GDYIEAVLDR NLAENISRVL YPNDNFFEGK ELRLKQEYFV
VAATLQDIIR RFKSSKFGCR DPVRTCFETF PDKVAIQLND THPALSIPEL
MRILVDVEKV DWDKAWEITK KTCAYTNHTV LPEALERWPV SMFEKLLPRH
LEIIYAINQR HLDHVAALFP GDVDRLRRMS VIEEGDCKRI NMAHLCVIGS
HAVNGVARIH SEIVKQSVFK DFYELEPEKF QNKTNGITPR RWLLLCNPGL
ADTIVEKIGE EFLTDLSQLK KLLPLVSDEV FIRDVAKVKQ ENKLKFSAFL
EKEYKVKINP SSMFDVHVKR IHEYKRQLLN CLHVVTLYNR IKRDPAKAFV
PRTVMIGGKA APGYHMAKLI IKLVTSIGDV VNHDPVVGDR LKVIFLENYR
VSLAEKVIPA ADLSQQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM
AEEAGAENLF IFGLRVEDVE ALDRKGYNAR EYYDHLPELK QAVDQISSGF
FSPKEPDCFK DIVNMLMHHD RFKVFADYEA YMQCQAQVDQ LYRNPKEWTK
KVIRNIACSG KFSSDRTITE YAREIWGVEP SDLQIPPPNI PRD
UCSC uc002wup.4; human.;

Category

Human PYGB ELISA Kit

Human GPBB ELISA Kit;Human glycogen phosphorylase B ELISA Kit;Human glycogen phosphorylase, brain form ELISA Kit;Human phosphorylase, glycogen; brain ELISA Kit;