Human RSPO2 ELISA Kit

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  • Alternative name

    Human roof plate-specific spondin-2 ELISA Kit;Human hRspo2 ELISA Kit;Human CRISTIN2 ELISA Kit;Human R-spondin 2 ELISA Kit;Human R-spondin-2 ELISA Kit;Human R-spondin 2 homolog ELISA Kit;

  • Catalog
    E032613
  • species
    Human
  • GeneRSPO2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of R-Spondin 2 (RSPO2). No significant cross-reactivity or interference between R-Spondin 2 (RSPO2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 6.2pg/mL.
  • Intended UseHuman RSPO2 ELISA Kit allows for the in vitro quantitative determination of RSPO2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to R-Spondin 2 (RSPO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to R-Spondin 2 (RSPO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain R-Spondin 2 (RSPO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of R-Spondin 2 (RSPO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human R-spondin-2 Protein information
  • Uniprot ID RSPO2_HUMAN
  • Uniprot AC Q6UXX9; B3KVP0; Q4G0U4; Q8N6X6;
  • UniGene Hs.444834;
  • GeneID 340419
  • KEGG hsa:340419;
  • Human R-spondin-2 Protein SEQUENCE
  • SEQUENCE 243 AA; 28315 MW; 96F44F105B21BB52 CRC64;

    MQFRLFSFAL IILNCMDYSH CQGNRWRRSK RASYVSNPIC KGCLSCSKDN

    GCSRCQQKLF FFLRREGMRQ YGECLHSCPS GYYGHRAPDM NRCARCRIEN

    CDSCFSKDFC TKCKVGFYLH RGRCFDECPD GFAPLEETME CVEGCEVGHW

    SEWGTCSRNN RTCGFKWGLE TRTRQIVKKP VKDTILCPTI AESRRCKMTM

    RHCPGGKRTP KAKEKRNKKK KRKLIERAQE QHSVFLATDR ANQ

  • UCSC uc003ymq.4; human. [Q6UXX9-1];



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