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Human RAB27B ELISA Kit

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  • Alternative name

    Human C25KG ELISA Kit;Human RAB27B, member RAS oncogene family ELISA Kit;Human ras-related protein Rab-27B ELISA Kit;
  • Catalog
    E032669
  • species
    Human
  • GeneRAB27B
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human RAB27B. No significant cross reactivity or interference between human RAB27B and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human RAB27B is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
  • Intended UseHuman RAB27B ELISA Kit allows for the in vitro quantitative determination of RAB27B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RAB27B has been pre coated onto a microplate. Standards and samples are pipetted into the wells and any RAB27B present is bound by the immobilized antibody. After removing any unbound substances, a biotin conjugated antibody specific for RAB27B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAB27B bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Ras-related protein Rab-27B Protein information
  • Uniprot ID RB27B_HUMAN
  • Uniprot AC O00194; B2RAB0; Q9BZB6;
  • UniGene Hs.25318;
  • GeneID 5874
  • KEGG hsa:5874;
  • Human Ras-related protein Rab-27B Protein SEQUENCE

  • SEQUENCE 218 AA; 24608 MW; 8ED640F0C15EDCD3 CRC64;

    MTDGDYDYLI KLLALGDSGV GKTTFLYRYT DNKFNPKFIT TVGIDFREKR

    VVYNAQGPNG SSGKAFKVHL QLWDTAGQER FRSLTTAFFR DAMGFLLMFD

    LTSQQSFLNV RNWMSQLQAN AYCENPDIVL IGNKADLPDQ REVNERQARE

    LADKYGIPYF ETSAATGQNV EKAVETLLDL IMKRMEQCVE KTQIPDTVNG

    GNSGNLDGEK PPEKKCIC

  • UCSC uc002lfr.4; human.;



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