Human RAB5A ELISA Kit

Alternative name
Human RAB5 ELISA Kit;Human RAB5A, member RAS oncogene family ELISA Kit;Human ras-related protein Rab-5A ELISA Kit;Human RAS-associated protein RAB5A ELISA Kit;
Catalog
E032703
species
Human
GeneRAB5A
SpecificityThis assay has high sensitivity and excellent specificity for detection of RAB5A. No significant cross-reactivity or interference between RAB5A and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human RAB5A is typically less than 0.122ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
Intended UseHuman RAB5A ELISA Kit allows for the in vitro quantitative determination of RAB5A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAB5A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAB5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAB5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RAB5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Ras-related protein Rab-5A Protein information
Uniprot ID RAB5A_HUMAN
Uniprot AC P20339; B4DJA5; Q6FI44;
UniGene Hs.475663;
GeneID 5868
KEGG hsa:5868;
Human Ras-related protein Rab-5A Protein SEQUENCE
SEQUENCE 215 AA; 23659 MW; EC03DDF96BBEF821 CRC64;
MASRGATRPN GPNTGNKICQ FKLVLLGESA VGKSSLVLRF VKGQFHEFQE
STIGAAFLTQ TVCLDDTTVK FEIWDTAGQE RYHSLAPMYY RGAQAAIVVY
DITNEESFAR AKNWVKELQR QASPNIVIAL SGNKADLANK RAVDFQEAQS
YADDNSLLFM ETSAKTSMNV NEIFMAIAKK LPKNEPQNPG ANSARGRGVD
LTEPTQPTRN QCCSN
UCSC uc003cbn.4; human. [P20339-1];

Category

Human RAB5A ELISA Kit

Human RAB5 ELISA Kit;Human RAB5A, member RAS oncogene family ELISA Kit;Human ras-related protein Rab-5A ELISA Kit;Human RAS-associated protein RAB5A ELISA Kit;