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Human RFTN1 ELISA Kit

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  • Alternative name

    Human cell migration-inducing gene 2 protein ELISA Kit;Human raft-linking protein ELISA Kit;Human KIAA0084 ELISA Kit;Human MIG2 ELISA Kit;Human PIB10 ELISA Kit;Human PIG9 ELISA Kit;Human raftlin ELISA Kit;Human raftlin, lipid raft linker 1 ELISA Kit;Human proliferation-inducing protein 10 ELISA Kit;Human proliferation-inducing protein 9 ELISA Kit;
  • Catalog
    E032768
  • species
    Human
  • GeneRFTN1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RFTN1. No significant cross-reactivity or interference between RFTN1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RFTN1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman RFTN1 ELISA Kit allows for the in vitro quantitative determination of RFTN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: This RFTN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RFTN1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||RFTN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RFTN1 antibody and an RFTN1-HRP conjugate. The assay sample and buffer are incubated together with RFTN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RFTN1 concentration since RFTN1 from samples and RFTN1-HRP conjugate compete for the anti-RFTN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by RFTN1 from the sample, fewer sites are left to bind RFTN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RFTN1 concentration in each sample is interpolated from this standard curve.
  • Human Raftlin Protein information
  • Uniprot ID RFTN1_HUMAN
  • Uniprot AC Q14699; Q0D2G0; Q496Y2; Q4QQI7; Q5JB48; Q7Z7P2;
  • UniGene Hs.98910;
  • GeneID 23180
  • KEGG hsa:23180;
  • Human Raftlin Protein SEQUENCE

  • SEQUENCE 578 AA; 63146 MW; 889928D62A62F189 CRC64;

    MGCGLNKLEK RDEKRPGNIY STLKRPQVET KIDVSYEYRF LEFTTLSAAE

    LPGSSAVRLA SLRDLPAQLL ELYQQGFSLA ALHPFVQPTH EREKTPLEHI

    FRAILIKKTD RSQKTDLHNE GYILELDCCS SLDHPTDQKL IPEFIKKIQE

    AASQGLKFVG VIPQYHSSVN SAGSSAPVST ANSTEDARDA KNARGDHASL

    ENEKPGTGDV CSAPAGRNQS PEPSSGPRGE VPLAKQPSSP SGEGDGGELS

    PQGVSKTLDG PESNPLEVHE EPLSGKMEIF TLFNKPKSHQ KCRQYYPVTI

    PLHVSKNGQT VSGLDANWLE HMSDHFRKGG MLVNAVFYLG IVNDSLHGLT

    DGVFIFEAVS TEDSKTIQGY DAIVVEQWTV LEGVEVQTDY VPLLNSLAAY

    GWQLTCVLPT PVVKTTSEGS VSTKQIVFLQ RPCLPQKIKK KESKFQWRFS

    REEMHNRQMR KSKGKLSARD KQQAEENEKN LEDQSSKAGD MGNCVSGQQQ

    EGGVSEEMKG PVQEDKGEQL SPGGLLCGVG VEGEAVQNGP ASHSRALVGI

    CTGHSNPGED ARDGDAEEVR ELGTVEEN

  • UCSC uc003cay.4; human.;



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