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Human RAPGEF4 ELISA Kit

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  • Alternative name

    Human exchange factor directly activated by cAMP 2 ELISA Kit;Human exchange protein directly activated by cAMP 2 ELISA Kit;Human EPAC 2 ELISA Kit;Human cAMP-regulated guanine nucleotide exchange factor II ELISA Kit;Human CAMP-GEFII ELISA Kit;Human CGEF2 ELISA Kit;Human EPAC2 ELISA Kit;Human EPAC ELISA Kit;Human Nbla00496 ELISA Kit;Human rap guanine nucleotide exchange factor 4 ELISA Kit;Human RAP guanine-nucleotide-exchange factor (GEF) 4 ELISA Kit;Human Rap guanine nucleotide exchange factor (GEF) 4 ELISA Kit;Human putative protein product of Nbla00496 ELISA Kit;
  • Catalog
    E032813
  • species
    Human
  • GeneRAPGEF4
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human RAPGEF4. No significant cross-reactivity or interference between human RAPGEF4 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human RAPGEF4 is typically less than 6.25pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
  • Intended UseHuman RAPGEF4 ELISA Kit allows for the in vitro quantitative determination of RAPGEF4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RAPGEF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAPGEF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAPGEF4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAPGEF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Rap guanine nucleotide exchange factor 4 Protein information
  • Uniprot ID RPGF4_HUMAN
  • Uniprot AC Q8WZA2; B2R7R3; B7Z283; B7Z3T6; B7Z912; O95636; Q8IXK6; Q8TAA4;
  • UniGene Hs.470646;
  • GeneID 11069
  • KEGG hsa:11069;
  • Human Rap guanine nucleotide exchange factor 4 Protein SEQUENCE

  • SEQUENCE 1011 AA; 115522 MW; 9427C5F92F2FFAD1 CRC64;

    MVAAHAAHSS SSAEWIACLD KRPLERSSED VDIIFTRLKE VKAFEKFHPN

    LLHQICLCGY YENLEKGITL FRQGDIGTNW YAVLAGSLDV KVSETSSHQD

    AVTICTLGIG TAFGESILDN TPRHATIVTR ESSELLRIEQ KDFKALWEKY

    RQYMAGLLAP PYGVMETGSN NDRIPDKENT PLIEPHVPLR PANTITKVPS

    EKILRAGKIL RNAILSRAPH MIRDRKYHLK TYRQCCVGTE LVDWMMQQTP

    CVHSRTQAVG MWQVLLEDGV LNHVDQEHHF QDKYLFYRFL DDEHEDAPLP

    TEEEKKECDE ELQDTMLLLS QMGPDAHMRM ILRKPPGQRT VDDLEIIYEE

    LLHIKALSHL STTVKRELAG VLIFESHAKG GTVLFNQGEE GTSWYIILKG

    SVNVVIYGKG VVCTLHEGDD FGKLALVNDA PRAASIVLRE DNCHFLRVDK

    EDFNRILRDV EANTVRLKEH DQDVLVLEKV PAGNRASNQG NSQPQQKYTV

    MSGTPEKILE HFLETIRLEA TLNEATDSVL NDFIMMHCVF MPNTQLCPAL

    VAHYHAQPSQ GTEQEKMDYA LNNKRRVIRL VLQWAAMYGD LLQEDDVSMA

    FLEEFYVSVS DDARMIAALK EQLPELEKIV KQISEDAKAP QKKHKVLLQQ

    FNTGDERAQK RQPIRGSDEV LFKVYCMDHT YTTIRVPVAT SVKEVISAVA

    DKLGSGEGLI IVKMSSGGEK VVLKPNDVSV FTTLTINGRL FACPREQFDS

    LTPLPEQEGP TVGTVGTFEL MSSKDLAYQM TIYDWELFNC VHELELIYHT

    FGRHNFKKTT ANLDLFLRRF NEIQFWVVTE ICLCSQLSKR VQLLKKFIKI

    AAHCKEYKNL NSFFAIVMGL SNVAVSRLAL TWEKLPSKFK KFYAEFESLM

    DPSRNHRAYR LTVAKLEPPL IPFMPLLIKD MTFTHEGNKT FIDNLVNFEK

    MRMIANTART VRYYRSQPFN PDAAQANKNH QDVRSYVRQL NVIDNQRTLS

    QMSHRLEPRR P

  • UCSC uc002uhv.5; human. [Q8WZA2-1];



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