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Human RAP1B ELISA Kit

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  • Alternative name

    Human GTP-binding protein smg p21B ELISA Kit;Human K-REV ELISA Kit;Human RAL1B ELISA Kit;Human RAP1B, member of RAS oncogene family ELISA Kit;Human ras-related protein Rap-1b ELISA Kit;Human RAS-related protein RAP1B ELISA Kit;Human Ras family small GTP binding protein RAP1B ELISA Kit;Human small GTP binding protein ELISA Kit;
  • Catalog
    E032823
  • species
    Human
  • GeneRAP1B
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human RAP1B. No significant cross-reactivity or interference between human RAP1B and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human RAP1B is typically less than 5.86 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman RAP1B ELISA Kit allows for the in vitro quantitative determination of RAP1B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RAP1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAP1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAP1B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAP1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Ras-related protein Rap-1b Protein information
  • Uniprot ID RAP1B_HUMAN
  • Uniprot AC Q6DCA1; Q6LES0;
  • UniGene Hs.369920;
  • GeneID 5908
  • KEGG hsa:5908;
  • Human Ras-related protein Rap-1b Protein SEQUENCE

  • SEQUENCE 184 AA; 20825 MW; CE976895E5965224 CRC64;

    MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ

    CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI

    LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS

    KINVNEIFYD LVRQINRKTP VPGKARKKSS CQLL

  • UCSC uc001sub.5; human. [P61224-1];



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