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Human RIPK3 ELISA Kit

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  • Alternative name

    Human RIP-like protein kinase 3 ELISA Kit;Human Receptor-interacting protein 3 ELISA Kit;Human RIP-3 ELISA Kit;Human RIP3 ELISA Kit;Human receptor interacting serine/threonine kinase 3 ELISA Kit;Human receptor-interacting serine/threonine-protein kinase 3 ELISA Kit;Human receptor interacting protein 3 ELISA Kit;
  • Catalog
    E033009
  • species
    Human
  • GeneRIPK3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Receptor Interacting Serine Threonine Kinase 3 (RIPK3). No significant cross-reactivity or interference between Receptor Interacting Serine Threonine Kinase 3 (RIPK3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.122ng/mL.
  • Intended UseHuman RIPK3 ELISA Kit allows for the in vitro quantitative determination of RIPK3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Receptor Interacting Serine Threonine Kinase 3 (RIPK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Receptor Interacting Serine Threonine Kinase 3 (RIPK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Receptor Interacting Serine Threonine Kinase 3 (RIPK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Receptor Interacting Serine Threonine Kinase 3 (RIPK3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Receptor-interacting serine/threonine-protein kinase 3 Protein information
  • Uniprot ID RIPK3_HUMAN
  • Uniprot AC Q9Y572; B4DJL9; C4AM87; Q5J795; Q5J796; Q6P5Y1;
  • UniGene Hs.268551;
  • GeneID 11035
  • KEGG hsa:11035;
  • Human Receptor-interacting serine/threonine-protein kinase 3 Protein SEQUENCE

  • SEQUENCE 518 AA; 56887 MW; BF755F8A0B1810A1 CRC64;

    MSCVKLWPSG APAPLVSIEE LENQELVGKG GFGTVFRAQH RKWGYDVAVK

    IVNSKAISRE VKAMASLDNE FVLRLEGVIE KVNWDQDPKP ALVTKFMENG

    SLSGLLQSQC PRPWPLLCRL LKEVVLGMFY LHDQNPVLLH RDLKPSNVLL

    DPELHVKLAD FGLSTFQGGS QSGTGSGEPG GTLGYLAPEL FVNVNRKAST

    ASDVYSFGIL MWAVLAGREV ELPTEPSLVY EAVCNRQNRP SLAELPQAGP

    ETPGLEGLKE LMQLCWSSEP KDRPSFQECL PKTDEVFQMV ENNMNAAVST

    VKDFLSQLRS SNRRFSIPES GQGGTEMDGF RRTIENQHSR NDVMVSEWLN

    KLNLEEPPSS VPKKCPSLTK RSRAQEEQVP QAWTAGTSSD SMAQPPQTPE

    TSTFRNQMPS PTSTGTPSPG PRGNQGAERQ GMNWSCRTPE PNPVTGRPLV

    NIYNCSGVQV GDNNYLTMQQ TTALPTWGLA PSGKGRGLQH PPPVGSQEGP

    KDPEAWSRPQ GWYNHSGK

  • UCSC uc001wpb.4; human. [Q9Y572-1];



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