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Alternative name
Regenerating islet-derived protein 3-gamma ELISA KIT; Pancreatitis-associated protein 1B ELISA KIT; PAP-1B ELISA KIT; Pancreatitis-associated protein IB ELISA KIT; PAP IB ELISA KIT; Regenerating islet-derived protein III-gamma ELISA KIT; REG III ELISA KIT; Reg III-gammaREG3G ELISA KIT; PAP1B ELISA KIT; REG-3-gamma ELISA KIT; PAP-1B ELISA KIT; PAP IB ELISA KIT; REG III ELISA KIT; Reg III-gamma ELISA KIT
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Catalog
E033055
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species
Human
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GeneREG3gamma
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Human REG3gamma. No significant cross-reactivity or interference between Human REG3gamma and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human REG3gamma and all the analogues, therefore, cross reaction may still exist.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.938ng/mL
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Intended UseHuman REG3gamma ELISA Kit allows for the in vitro quantitative determination of REG3gamma , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of REG3gamma in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human REG3gamma. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human REG3gamma and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human REG3gamma, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human REG3gamma. You can calculate the concentration of Human REG3gamma in the samples by comparing the OD of the samples to the standard curve.
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