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Catalog
E033072
species
Human
GeneREG3G
SpecificityThis assay has high sensitivity and excellent specificity for detection of Regenerating Islet Derived Protein 3 Gamma (REG3g).
No significant cross-reactivity or interference between Regenerating Islet Derived Protein 3 Gamma (REG3g) and analogues was observed.
Intended UseHuman REG3G ELISA Kit allows for the in vitro quantitative determination of REG3G , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 3 Gamma (REG3g). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 3 Gamma (REG3g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 3 Gamma (REG3g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Regenerating Islet Derived Protein 3 Gamma (REG3g) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Regenerating islet-derived protein 3-gamma 15 kDa form Protein information
Uniprot ID
REG3G_HUMAN
Uniprot AC
Q6UW15; A8K980; D6W5J6; Q3SYE4; Q3SYE6; Q6FH18;
UniGene
Hs.447084;
GeneID
130120
KEGG
hsa:130120;
Human Regenerating islet-derived protein 3-gamma 15 kDa form Protein SEQUENCE