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Alternative name
Regenerating islet-derived protein 4 ELISA KIT; Gastrointestinal secretory protein ELISA KIT; REG-like protein ELISA KIT; Regenerating islet-derived protein IV ELISA KIT; Reg IVREG4 ELISA KIT; GISP ELISA KIT; RELP ELISA KIT; REG-4 ELISA KIT; Reg IV ELISA KIT
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Catalog
E033073
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species
Human
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GeneREG-4
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity<0.094ng/ml
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Intended UseHuman REG-4 ELISA Kit allows for the in vitro quantitative determination of REG-4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human REG4antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human REG4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human REG4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman REG4can be calculated.
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