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Alternative name
C-C motif chemokine 5 ELISA KIT; EoCP ELISA KIT; Eosinophil chemotactic cytokine ELISA KIT; SIS-delta ELISA KIT; Small-inducible cytokine A5 ELISA KIT; T cell-specific protein P228 ELISA KIT; TCP228 ELISA KIT; T-cell-specific protein RANTESCleaved into the following 2 chains:RANTES(3-68) ELISA KIT; RANTES(4-68)CCL5 ELISA KIT; D17S136E ELISA KIT; SCYA5 ELISA KIT; TCP228 ELISA KIT
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Catalog
E033088
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species
Human
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GeneRANTES
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SpecificityThis assay has high sensitivity and excellent specificity for detection of RANTES. No significant cross-reactivity or interference between RANTES and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RANTES and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL.
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Intended UseHuman RANTES ELISA Kit allows for the in vitro quantitative determination of RANTES , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyImmunology
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Product Description
specificalPrinciple of the assay: RANTES ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RANTES antibody and an RANTES-HRP conjugate. The assay sample and buffer are incubated together with RANTES-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RANTES concentration since RANTES from samples and RANTES-HRP conjugate compete for the anti-RANTES antibody binding site. Since the number of sites is limited, as more sites are occupied by RANTES from the sample, fewer sites are left to bind RANTES-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RANTES concentration in each sample is interpolated from this standard curve.
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