Human ROCK2 ELISA Kit

Alternative name
Human Rho kinase 2 ELISA Kit;Human Rho-associated, coiled-coil-containing protein kinase 2 ELISA Kit;Human rho-associated, coiled-coil-containing protein kinase II ELISA Kit;Human ROCK-II ELISA Kit;Human p164 ROCK-2 ELISA Kit;Human KIAA0619 ELISA Kit;Human Rho associated coiled-coil containing protein kinase 2 ELISA Kit;Human rho-associated protein kinase 2 ELISA Kit;
Catalog
E033479
species
Human
GeneROCK2
SpecificityThis assay has high sensitivity for detection of Rock2. 100% cross-reactivity of Rock2 was observed among human, mouse and rat.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.119ng/mL.
Intended UseHuman ROCK2 ELISA Kit allows for the in vitro quantitative determination of ROCK2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Rock2 in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Due to the 97% homology of the sequence among different species, the kit can be used to detect human, mouse and rat samples. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rock2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rock2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rock2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Rock2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Rho-associated protein kinase 2 Protein information
Uniprot ID ROCK2_HUMAN
Uniprot AC O75116; Q53QZ0; Q53SJ7; Q9UQN5;
UniGene Hs.681743;
GeneID 9475
KEGG hsa:9475;
Human Rho-associated protein kinase 2 Protein SEQUENCE
SEQUENCE 1388 AA; 160900 MW; 876240F410C2487E CRC64;
MSRPPPTGKM PGAPETAPGD GAGASRQRKL EALIRDPRSP INVESLLDGL
NSLVLDLDFP ALRKNKNIDN FLNRYEKIVK KIRGLQMKAE DYDVVKVIGR
GAFGEVQLVR HKASQKVYAM KLLSKFEMIK RSDSAFFWEE RDIMAFANSP
WVVQLFYAFQ DDRYLYMVME YMPGGDLVNL MSNYDVPEKW AKFYTAEVVL
ALDAIHSMGL IHRDVKPDNM LLDKHGHLKL ADFGTCMKMD ETGMVHCDTA
VGTPDYISPE VLKSQGGDGF YGRECDWWSV GVFLYEMLVG DTPFYADSLV
GTYSKIMDHK NSLCFPEDAE ISKHAKNLIC AFLTDREVRL GRNGVEEIRQ
HPFFKNDQWH WDNIRETAAP VVPELSSDID SSNFDDIEDD KGDVETFPIP
KAFVGNQLPF IGFTYYRENL LLSDSPSCRE TDSIQSRKNE ESQEIQKKLY
TLEEHLSNEM QAKEELEQKC KSVNTRLEKT AKELEEEITL RKSVESALRQ
LEREKALLQH KNAEYQRKAD HEADKKRNLE NDVNSLKDQL EDLKKRNQNS
QISTEKVNQL QRQLDETNAL LRTESDTAAR LRKTQAESSK QIQQLESNNR
DLQDKNCLLE TAKLKLEKEF INLQSALESE RRDRTHGSEI INDLQGRICG
LEEDLKNGKI LLAKVELEKR QLQERFTDLE KEKSNMEIDM TYQLKVIQQS
LEQEEAEHKA TKARLADKNK IYESIEEAKS EAMKEMEKKL LEERTLKQKV
ENLLLEAEKR CSLLDCDLKQ SQQKINELLK QKDVLNEDVR NLTLKIEQET
QKRCLTQNDL KMQTQQVNTL KMSEKQLKQE NNHLMEMKMN LEKQNAELRK
ERQDADGQMK ELQDQLEAEQ YFSTLYKTQV RELKEECEEK TKLGKELQQK
KQELQDERDS LAAQLEITLT KADSEQLARS IAEEQYSDLE KEKIMKELEI
KEMMARHKQE LTEKDATIAS LEETNRTLTS DVANLANEKE ELNNKLKDVQ
EQLSRLKDEE ISAAAIKAQF EKQLLTERTL KTQAVNKLAE IMNRKEPVKR
GNDTDVRRKE KENRKLHMEL KSEREKLTQQ MIKYQKELNE MQAQIAEESQ
IRIELQMTLD SKDSDIEQLR SQLQALHIGL DSSSIGSGPG DAEADDGFPE
SRLEGWLSLP VRNNTKKFGW VKKYVIVSSK KILFYDSEQD KEQSNPYMVL
DIDKLFHVRP VTQTDVYRAD AKEIPRIFQI LYANEGESKK EQEFPVEPVG
EKSNYICHKG HEFIPTLYHF PTNCEACMKP LWHMFKPPPA LECRRCHIKC
HKDHMDKKEE IIAPCKVYYD ISTAKNLLLL ANSTEEQQKW VSRLVKKIPK
KPPAPDPFAR SSPRTSMKIQ QNQSIRRPSR QLAPNKPS
UCSC uc002rbd.2; human.;

Category

Human ROCK2 ELISA Kit