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Human ARHGDIA ELISA Kit

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  • Alternative name

    Human Rho GDI 1 ELISA Kit;Human Rho-GDI alpha ELISA Kit;Human GDIA1 ELISA Kit;Human HEL-S-47e ELISA Kit;Human NPHS8 ELISA Kit;Human RHOGDI ELISA Kit;Human RHOGDI-1 ELISA Kit;Human Rho GDP dissociation inhibitor alpha ELISA Kit;Human rho GDP-dissociation inhibitor 1 ELISA Kit;Human GDP-dissociation inhibitor, aplysia RAS-related 1 ELISA Kit;Human Rho GDP dissociation inhibitor (GDI) alpha ELISA Kit;Human epididymis secretory sperm binding protein Li 47e ELISA Kit;
  • Catalog
    E033491
  • species
    Human
  • GeneARHGDIA
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Rho GDP Dissociation Inhibitor Alpha (ARHGDIa). No significant cross-reactivity or interference between Rho GDP Dissociation Inhibitor Alpha (ARHGDIa) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.066ng/mL.
  • Intended UseHuman ARHGDIA ELISA Kit allows for the in vitro quantitative determination of ARHGDIA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rho GDP Dissociation Inhibitor Alpha (ARHGDIa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Rho GDP Dissociation Inhibitor Alpha (ARHGDIa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rho GDP Dissociation Inhibitor Alpha (ARHGDIa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rho GDP Dissociation Inhibitor Alpha (ARHGDIa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Rho GDP-dissociation inhibitor 1 Protein information
  • Uniprot ID GDIR1_HUMAN
  • Uniprot AC P52565; A8MXW0; B2R5X1; B4DDD3; B4DUV9; Q6IBM5;
  • UniGene Hs.159161; Hs.599508; Hs.728006;
  • GeneID 396
  • KEGG hsa:396;
  • Human Rho GDP-dissociation inhibitor 1 Protein SEQUENCE

  • SEQUENCE 204 AA; 23207 MW; 59CB6F42E3B3BCCA CRC64;

    MAEQEPTAEQ LAQIAAENEE DEHSVNYKPP AQKSIQEIQE LDKDDESLRK

    YKEALLGRVA VSADPNVPNV VVTGLTLVCS SAPGPLELDL TGDLESFKKQ

    SFVLKEGVEY RIKISFRVNR EIVSGMKYIQ HTYRKGVKID KTDYMVGSYG

    PRAEEYEFLT PVEEAPKGML ARGSYSIKSR FTDDDKTDHL SWEWNLTIKK

    DWKD

  • UCSC uc021ufg.2; human. [P52565-1];



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