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Human ARHGAP21 ELISA Kit

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  • Alternative name

    Human rho GTPase-activating protein 10 ELISA Kit;Human rho-type GTPase-activating protein 21 ELISA Kit;Human ARHGAP10 ELISA Kit;Human KIAA1424 ELISA Kit;Human Rho GTPase activating protein 21 ELISA Kit;Human rho GTPase-activating protein 21 ELISA Kit;Human Rho-GTPase activating protein 10 ELISA Kit;
  • Catalog
    E033520
  • species
    Human
  • GeneARHGAP21
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 9.4pg/ml
  • Intended UseHuman ARHGAP21 ELISA Kit allows for the in vitro quantitative determination of ARHGAP21 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ARHGAP21 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ARHGAP21 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ARHGAP21 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ARHGAP21 can be calculated.
  • Human Rho GTPase-activating protein 21 Protein information
  • Uniprot ID RHG21_HUMAN
  • Uniprot AC Q5T5U3; Q0VF98; Q7Z3P7; Q8N3A2; Q8NI19; Q8TBV5; Q9P2C3;
  • UniGene Hs.524195;
  • GeneID 57584
  • KEGG hsa:57584;
  • Human Rho GTPase-activating protein 21 Protein SEQUENCE

  • SEQUENCE 1957 AA; 217331 MW; EEE781DED0166E69 CRC64;

    MATRRTGLSE GDGDKLKACE VSKNKDGKEQ SETVSLSEDE TFSWPGPKTV

    TLKRTSQGFG FTLRHFIVYP PESAIQFSYK DEENGNRGGK QRNRLEPMDT

    IFVKQVKEGG PAFEAGLCTG DRIIKVNGES VIGKTYSQVI ALIQNSDTTL

    ELSVMPKDED ILQVLQFTKD VTALAYSQDA YLKGNEAYSG NARNIPEPPP

    ICYPWLPSAP SAMAQPVEIS PPDSSLSKQQ TSTPVLTQPG RAYRMEIQVP

    PSPTDVAKSN TAVCVCNESV RTVIVPSEKV VDLLSNRNNH TGPSHRTEEV

    RYGVSEQTSL KTVSRTTSPP LSIPTTHLIH QPAGSRSLEP SGILLKSGNY

    SGHSDGISSS RSQAVEAPSV SVNHYSPNSH QHIDWKNYKT YKEYIDNRRL

    HIGCRTIQER LDSLRAASQS TTDYNQVVPN RTTLQGRRRS TSHDRVPQSV

    QIRQRSVSQE RLEDSVLMKY CPRSASQGAL TSPSVSFSNH RTRSWDYIEG

    QDETLENVNS GTPIPDSNGE KKQTYKWSGF TEQDDRRGIC ERPRQQEIHK

    SFRGSNFTVA PSVVNSDNRR MSGRGVGSVS QFKKIPPDLK TLQSNRNFQT

    TCGMSLPRGI SQDRSPLVKV RSNSLKAPST HVTKPSFSQK SFVSIKDQRP

    VNHLHQNSLL NQQTWVRTDS APDQQVETGK SPSLSGASAK PAPQSSENAG

    TSDLELPVSQ RNQDLSLQEA ETEQSDTLDN KEAVILREKP PSGRQTPQPL

    RHQSYILAVN DQETGSDTTC WLPNDARREV HIKRMEERKA SSTSPPGDSL

    ASIPFIDEPT SPSIDHDIAH IPASAVISAS TSQVPSIATV PPCLTTSAPL

    IRRQLSHDHE SVGPPSLDAQ PNSKTERSKS YDEGLDDYRE DAKLSFKHVS

    SLKGIKIADS QKSSEDSGSR KDSSSEVFSD AAKEGWLHFR PLVTDKGKRV

    GGSIRPWKQM YVVLRGHSLY LYKDKREQTT PSEEEQPISV NACLIDISYS

    ETKRKNVFRL TTSDCECLFQ AEDRDDMLAW IKTIQESSNL NEEDTGVTNR

    DLISRRIKEY NNLMSKAEQL PKTPRQSLSI RQTLLGAKSE PKTQSPHSPK

    EESERKLLSK DDTSPPKDKG TWRKGIPSIM RKTFEKKPTA TGTFGVRLDD

    CPPAHTNRYI PLIVDICCKL VEERGLEYTG IYRVPGNNAA ISSMQEELNK

    GMADIDIQDD KWRDLNVISS LLKSFFRKLP EPLFTNDKYA DFIEANRKED

    PLDRLKTLKR LIHDLPEHHY ETLKFLSAHL KTVAENSEKN KMEPRNLAIV

    FGPTLVRTSE DNMTHMVTHM PDQYKIVETL IQHHDWFFTE EGAEEPLTTV

    QEESTVDSQP VPNIDHLLTN IGRTGVSPGD VSDSATSDST KSKGSWGSGK

    DQYSRELLVS SIFAAASRKR KKPKEKAQPS SSEDELDNVF FKKENVEQCH

    NDTKEESKKE SETLGRKQKI IIAKENSTRK DPSTTKDEKI SLGKESTPSE

    EPSPPHNSKH NKSPTLSCRF AILKESPRSL LAQKSSHLEE TGSDSGTLLS

    TSSQASLARF SMKKSTSPET KHSEFLANVS TITSDYSTTS SATYLTSLDS

    SRLSPEVQSV AESKGDEADD ERSELISEGR PVETDSESEF PVFPTALTSE

    RLFRGKLQEV TKSSRRNSEG SELSCTEGSL TSSLDSRRQL FSSHKLIECD

    TLSRKKSARF KSDSGSLGDA KNEKEAPSLT KVFDVMKKGK STGSLLTPTR

    GESEKQEPTW KTKIADRLKL RPRAPADDMF GVGNHKVNAE TAKRKSIRRR

    HTLGGHRDAT EISVLNFWKV HEQSGERESE LSAVNRLKPK CSAQDLSISD

    WLARERLRTS TSDLSRGEIG DPQTENPSTR EIATTDTPLS LHCNTGSSSS

    TLASTNRPLL SIPPQSPDQI NGESFQNVSK NASSAANAQP HKLSETPGSK

    AEFHPCL

  • UCSC uc001isb.2; human. [Q5T5U3-1];



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