Human RNASEL ELISA Kit

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  • Alternative name

    Human 2-5A-dependent RNase ELISA Kit;Human ribonuclease 4 ELISA Kit;Human ribonuclease L ELISA Kit;Human RNase L ELISA Kit;Human RNS4 ELISA Kit;Human PRCA1 ELISA Kit;Human 2-5A-dependent ribonuclease ELISA Kit;Human 2',5'-oligoisoadenylate synthetase-dependent ELISA Kit;Human interferon-induced 2-5A-dependent RNase ELISA Kit;

  • Catalog
    E033623
  • species
    Human
  • GeneRNASEL
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Ribonuclease L (RNASEL). No significant cross-reactivity or interference between Ribonuclease L (RNASEL) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 6.8pg/mL.
  • Intended UseHuman RNASEL ELISA Kit allows for the in vitro quantitative determination of RNASEL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease L (RNASEL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease L (RNASEL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease L (RNASEL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribonuclease L (RNASEL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human 2-5A-dependent ribonuclease Protein information
  • Uniprot ID RN5A_HUMAN
  • Uniprot AC Q05823; Q5W0L2; Q6AI46;
  • UniGene Hs.518545;
  • GeneID 6041
  • KEGG hsa:6041;
  • Human 2-5A-dependent ribonuclease Protein SEQUENCE
  • SEQUENCE 741 AA; 83533 MW; 91385EA307E3CE1D CRC64;

    MESRDHNNPQ EGPTSSSGRR AAVEDNHLLI KAVQNEDVDL VQQLLEGGAN

    VNFQEEEGGW TPLHNAVQMS REDIVELLLR HGADPVLRKK NGATPFILAA

    IAGSVKLLKL FLSKGADVNE CDFYGFTAFM EAAVYGKVKA LKFLYKRGAN

    VNLRRKTKED QERLRKGGAT ALMDAAEKGH VEVLKILLDE MGADVNACDN

    MGRNALIHAL LSSDDSDVEA ITHLLLDHGA DVNVRGERGK TPLILAVEKK

    HLGLVQRLLE QEHIEINDTD SDGKTALLLA VELKLKKIAE LLCKRGASTD

    CGDLVMTARR NYDHSLVKVL LSHGAKEDFH PPAEDWKPQS SHWGAALKDL

    HRIYRPMIGK LKFFIDEKYK IADTSEGGIY LGFYEKQEVA VKTFCEGSPR

    AQREVSCLQS SRENSHLVTF YGSESHRGHL FVCVTLCEQT LEACLDVHRG

    EDVENEEDEF ARNVLSSIFK AVQELHLSCG YTHQDLQPQN ILIDSKKAAH

    LADFDKSIKW AGDPQEVKRD LEDLGRLVLY VVKKGSISFE DLKAQSNEEV

    VQLSPDEETK DLIHRLFHPG EHVRDCLSDL LGHPFFWTWE SRYRTLRNVG

    NESDIKTRKS ESEILRLLQP GPSEHSKSFD KWTTKINECV MKKMNKFYEK

    RGNFYQNTVG DLLKFIRNLG EHIDEEKHKK MKLKIGDPSL YFQKTFPDLV

    IYVYTKLQNT EYRKHFPQTH SPNKPQCDGA GGASGLASPG C

  • UCSC uc001gpk.4; human. [Q05823-1];



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