Human RNASET2 ELISA Kit

Alternative name
Human ribonuclease 6 ELISA Kit;Human RNASE6PL ELISA Kit;Human bA514O12.3 ELISA Kit;Human ribonuclease T2 ELISA Kit;
Catalog
E033632
species
Human
GeneRNASET2
SpecificityThis assay has high sensitivity and excellent specificity for detection of RNASET2. No significant cross-reactivity or interference between RNASET2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RNASET2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman RNASET2 ELISA Kit allows for the in vitro quantitative determination of RNASET2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: RNASET2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RNASET2 antibody and an RNASET2-HRP conjugate. The assay sample and buffer are incubated together with RNASET2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RNASET2 concentration since RNASET2 from samples and RNASET2-HRP conjugate compete for the anti-RNASET2 antibody binding site. Since the number of sites is limited, as more sites are occupied by RNASET2 from the sample, fewer sites are left to bind RNASET2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RNASET2 concentration in each sample is interpolated from this standard curve.
Human Ribonuclease T2 Protein information
Uniprot ID RNT2_HUMAN
Uniprot AC O00584; B2RDA7; E1P5C3; Q5T8Q0; Q8TCU2; Q9BZ46; Q9BZ47;
UniGene Hs.529989;
GeneID 8635
KEGG hsa:8635;
Human Ribonuclease T2 Protein SEQUENCE
SEQUENCE 256 AA; 29481 MW; 7C8BB08B8ED853EB CRC64;
MRPAALRGAL LGCLCLALLC LGGADKRLRD NHEWKKLIMV QHWPETVCEK
IQNDCRDPPD YWTIHGLWPD KSEGCNRSWP FNLEEIKDLL PEMRAYWPDV
IHSFPNRSRF WKHEWEKHGT CAAQVDALNS QKKYFGRSLE LYRELDLNSV
LLKLGIKPSI NYYQVADFKD ALARVYGVIP KIQCLPPSQD EEVQTIGQIE
LCLTKQDQQL QNCTEPGEQP SPKQEVWLAN GAAESRGLRV CEDGPVFYPP
PKKTKH
UCSC uc003qve.4; human. [O00584-1];

Category

Human RNASET2 ELISA Kit

Human ribonuclease 6 ELISA Kit;Human RNASE6PL ELISA Kit;Human bA514O12.3 ELISA Kit;Human ribonuclease T2 ELISA Kit;