Human RPN1 ELISA Kit

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  • Alternative name

    Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit ELISA Kit;Human ribophorin I ELISA Kit;Human RPN-I ELISA Kit;Human ribophorin-1 ELISA Kit;Human OST1 ELISA Kit;Human RBPH1 ELISA Kit;Human dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 ELISA Kit;Human dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit ELISA Kit;Human oligosaccharyltransferase 1 homolog ELISA Kit;Human oligosaccharyltransferase complex subunit (non-catalytic) ELISA Kit;

  • Catalog
    E033660
  • species
    Human
  • GeneRPN1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Ribophorin I (RPN1). No significant cross-reactivity or interference between Ribophorin I (RPN1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.63ng/mL.
  • Intended UseHuman RPN1 ELISA Kit allows for the in vitro quantitative determination of RPN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribophorin I (RPN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribophorin I (RPN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribophorin I (RPN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribophorin I (RPN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 Protein information
  • Uniprot ID RPN1_HUMAN
  • Uniprot AC P04843; B2R5Z0; D3DNB6; Q68DT1;
  • UniGene Hs.518244; Hs.603636;
  • GeneID 6184
  • KEGG hsa:6184;
  • Human Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 Protein SEQUENCE
  • SEQUENCE 607 AA; 68569 MW; A2351A9CFABAEB6C CRC64;

    MEAPAAGLFL LLLLGTWAPA PGSASSEAPP LINEDVKRTV DLSSHLAKVT

    AEVVLAHLGG GSTSRATSFL LALEPELEAR LAHLGVQVKG EDEEENNLEV

    RETKIKGKSG RFFTVKLPVA LDPGAKISVI VETVYTHVLH PYPTQITQSE

    KQFVVFEGNH YFYSPYPTKT QTMRVKLASR NVESYTKLGN PTRSEDLLDY

    GPFRDVPAYS QDTFKVHYEN NSPFLTITSM TRVIEVSHWG NIAVEENVDL

    KHTGAVLKGP FSRYDYQRQP DSGISSIRSF KTILPAAAQD VYYRDEIGNV

    STSHLLILDD SVEMEIRPRF PLFGGWKTHY IVGYNLPSYE YLYNLGDQYA

    LKMRFVDHVF DEQVIDSLTV KIILPEGAKN IEIDSPYEIS RAPDELHYTY

    LDTFGRPVIV AYKKNLVEQH IQDIVVHYTF NKVLMLQEPL LVVAAFYILF

    FTVIIYVRLD FSITKDPAAE ARMKVACITE QVLTLVNKRI GLYRHFDETV

    NRYKQSRDIS TLNSGKKSLE TEHKALTSEI ALLQSRLKTE GSDLCDRVSE

    MQKLDAQVKE LVLKSAVEAE RLVAGKLKKD TYIENEKLIS GKRQELVTKI

    DHILDAL

  • UCSC uc003ekr.2; human.;



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