Human RPIA ELISA Kit

Alternative name
Human phosphoriboisomerase ELISA Kit;Human RPI ELISA Kit;Human RPIAD ELISA Kit;Human ribose 5-phosphate isomerase A ELISA Kit;Human ribose-5-phosphate isomerase ELISA Kit;Human ribose 5-phosphate epimerase ELISA Kit;
Catalog
E033665
species
Human
GeneRPIA
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.938ng/ml
Intended UseHuman RPIA ELISA Kit allows for the in vitro quantitative determination of RPIA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human RPIA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human RPIA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human RPIA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human RPIA can be calculated.
Human Ribose-5-phosphate isomerase Protein information
Uniprot ID RPIA_HUMAN
Uniprot AC P49247; Q541P9; Q96BJ6;
UniGene Hs.469264;
GeneID 22934
KEGG hsa:22934;
Human Ribose-5-phosphate isomerase Protein SEQUENCE
SEQUENCE 311 AA; 33269 MW; 4ED587A40F10223D CRC64;
MQRPGPFSTL YGRVLAPLPG RAGGAASGGG GNSWDLPGSH VRLPGRAQSG
TRGGAGNTST SCGDSNSICP APSTMSKAEE AKKLAGRAAV ENHVRNNQVL
GIGSGSTIVH AVQRIAERVK QENLNLVCIP TSFQARQLIL QYGLTLSDLD
RHPEIDLAID GADEVDADLN LIKGGGGCLT QEKIVAGYAS RFIVIADFRK
DSKNLGDQWH KGIPIEVIPM AYVPVSRAVS QKFGGVVELR MAVNKAGPVV
TDNGNFILDW KFDRVHKWSE VNTAIKMIPG VVDTGLFINM AERVYFGMQD
GSVNMREKPF C
UCSC uc002ste.4; human.;

Category

Human RPIA ELISA Kit

Human phosphoriboisomerase ELISA Kit;Human RPI ELISA Kit;Human RPIAD ELISA Kit;Human ribose 5-phosphate isomerase A ELISA Kit;Human ribose-5-phosphate isomerase ELISA Kit;Human ribose 5-phosphate epimerase ELISA Kit;