Human RPS6KB1 ELISA Kit

Alternative name
Human S6K-beta-1 ELISA Kit;Human S6K1 ELISA Kit;Human 70 kDa ribosomal protein S6 kinase 1 ELISA Kit;Human P70S6K1 ELISA Kit;Human p70-S6K 1 ELISA Kit;Human ribosomal protein S6 kinase I ELISA Kit;Human serine/threonine-protein kinase 14A ELISA Kit;Human p70 ribosomal S6 kinase alpha ELISA Kit;Human p70 S6 kinase alpha ELISA Kit;Human p70 S6K-alpha ELISA Kit;Human p70 S6KA ELISA Kit;Human STK14A ELISA Kit;Human PS6K ELISA Kit;Human S6K ELISA Kit;Human p70(S6K)-alpha ELISA Kit;Human p70-S6K ELISA Kit;Human p70-alpha ELISA Kit;Human ribosomal protein S6 kinase B1 ELISA Kit;Human ribosomal protein S6 kinase beta-1 ELISA Kit;Human p70 S6 kinase, alpha ELISA Kit;Human ribosomal protein S6 kinase, 70kDa, polypeptide 1 ELISA Kit;Human serine/threonine kinase 14 alpha ELISA Kit;
Catalog
E033708
species
Human
GeneRPS6KB1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1). No significant cross-reactivity or interference between Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.058ng/mL.
Intended UseHuman RPS6KB1 ELISA Kit allows for the in vitro quantitative determination of RPS6KB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribosomal Protein S6 Kinase Beta 1 (RPS6Kb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Ribosomal protein S6 kinase beta-1 Protein information
Uniprot ID KS6B1_HUMAN
Uniprot AC P23443; B2R779; B4DLT4; B4DTG1; E7ESB8; F6UYM1; Q7Z721;
UniGene Hs.463642;
GeneID 6198
KEGG hsa:6198;
Human Ribosomal protein S6 kinase beta-1 Protein SEQUENCE
SEQUENCE 525 AA; 59140 MW; 2C3BA13CCDAF4AB3 CRC64;
MRRRRRRDGF YPAPDFRDRE AEDMAGVFDI DLDQPEDAGS EDELEEGGQL
NESMDHGGVG PYELGMEHCE KFEISETSVN RGPEKIRPEC FELLRVLGKG
GYGKVFQVRK VTGANTGKIF AMKVLKKAMI VRNAKDTAHT KAERNILEEV
KHPFIVDLIY AFQTGGKLYL ILEYLSGGEL FMQLEREGIF MEDTACFYLA
EISMALGHLH QKGIIYRDLK PENIMLNHQG HVKLTDFGLC KESIHDGTVT
HTFCGTIEYM APEILMRSGH NRAVDWWSLG ALMYDMLTGA PPFTGENRKK
TIDKILKCKL NLPPYLTQEA RDLLKKLLKR NAASRLGAGP GDAGEVQAHP
FFRHINWEEL LARKVEPPFK PLLQSEEDVS QFDSKFTRQT PVDSPDDSTL
SESANQVFLG FTYVAPSVLE SVKEKFSFEP KIRSPRRFIG SPRTPVSPVK
FSPGDFWGRG ASASTANPQT PVEYPMETSG IEQMDVTMSG EASAPLPIRQ
PNSGPYKKQA FPMISKRPEH LRMNL
UCSC uc002ixy.5; human. [P23443-1];

Category

Human RPS6KB1 ELISA Kit