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Human RNF4 ELISA Kit

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  • Alternative name

    Human ring finger protein 4 ELISA Kit;Human RING-type E3 ubiquitin transferase RNF4 ELISA Kit;Human small nuclear RING finger protein ELISA Kit;Human Protein SNURF ELISA Kit;Human SNURF ELISA Kit;Human RES4-26 ELISA Kit;Human SLX5 ELISA Kit;Human E3 ubiquitin-protein ligase RNF4 ELISA Kit;Human E3 ubiquitin ligase RNF4 ELISA Kit;
  • Catalog
    E033821
  • species
    Human
  • GeneRNF4
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman RNF4 ELISA Kit allows for the in vitro quantitative determination of RNF4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the Assay: RNF4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for RNF4. Standards or samples are then added to the microtiter plate wells and RNF4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of RNF4 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for RNF4 are added to each well to "sandwich" the RNF4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain RNF4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RNF4 concentration in each sample is interpolated from this standard curve.
  • Human E3 ubiquitin-protein ligase RNF4 Protein information
  • Uniprot ID RNF4_HUMAN
  • Uniprot AC P78317; B2R6D6; D6RF58; Q49AR8;
  • UniGene Hs.66394;
  • GeneID 6047
  • KEGG hsa:6047;
  • Human E3 ubiquitin-protein ligase RNF4 Protein SEQUENCE

  • SEQUENCE 190 AA; 21319 MW; E5E3AE4A9B28CF9D CRC64;

    MSTRKRRGGA INSRQAQKRT REATSTPEIS LEAEPIELVE TAGDEIVDLT

    CESLEPVVVD LTHNDSVVIV DERRRPRRNA RRLPQDHADS CVVSSDDEEL

    SRDRDVYVTT HTPRNARDEG ATGLRPSGTV SCPICMDGYS EIVQNGRLIV

    STECGHVFCS QCLRDSLKNA NTCPTCRKKI NHKRYHPIYI

  • UCSC uc003gfb.4; human. [P78317-1];



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