Human ROBO1 ELISA Kit

Alternative name
Human deleted in U twenty twenty ELISA Kit;Human H-Robo-1 ELISA Kit;Human DUTT1 ELISA Kit;Human SAX3 ELISA Kit;Human roundabout guidance receptor 1 ELISA Kit;Human roundabout homolog 1 ELISA Kit;Human roundabout, axon guidance receptor, homolog 1 ELISA Kit;
Catalog
E033971
species
Human
GeneROBO1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1). No significant cross-reactivity or interference between Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.118ng/mL.
Intended UseHuman ROBO1 ELISA Kit allows for the in vitro quantitative determination of ROBO1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Roundabout homolog 1 Protein information
Uniprot ID ROBO1_HUMAN
Uniprot AC Q9Y6N7; B2RXI1; D3DU36; E9PD49; Q1RMC7; Q7Z300; Q9BUS7;
UniGene Hs.744218;
GeneID 6091
KEGG hsa:6091;
Human Roundabout homolog 1 Protein SEQUENCE
SEQUENCE 1651 AA; 180930 MW; 9D98CD7CAB73074D CRC64;
MKWKHVPFLV MISLLSLSPN HLFLAQLIPD PEDVERGNDH GTPIPTSDND
DNSLGYTGSR LRQEDFPPRI VEHPSDLIVS KGEPATLNCK AEGRPTPTIE
WYKGGERVET DKDDPRSHRM LLPSGSLFFL RIVHGRKSRP DEGVYVCVAR
NYLGEAVSHN ASLEVAILRD DFRQNPSDVM VAVGEPAVME CQPPRGHPEP
TISWKKDGSP LDDKDERITI RGGKLMITYT RKSDAGKYVC VGTNMVGERE
SEVAELTVLE RPSFVKRPSN LAVTVDDSAE FKCEARGDPV PTVRWRKDDG
ELPKSRYEIR DDHTLKIRKV TAGDMGSYTC VAENMVGKAE ASATLTVQEP
PHFVVKPRDQ VVALGRTVTF QCEATGNPQP AIFWRREGSQ NLLFSYQPPQ
SSSRFSVSQT GDLTITNVQR SDVGYYICQT LNVAGSIITK AYLEVTDVIA
DRPPPVIRQG PVNQTVAVDG TFVLSCVATG SPVPTILWRK DGVLVSTQDS
RIKQLENGVL QIRYAKLGDT GRYTCIASTP SGEATWSAYI EVQEFGVPVQ
PPRPTDPNLI PSAPSKPEVT DVSRNTVTLS WQPNLNSGAT PTSYIIEAFS
HASGSSWQTV AENVKTETSA IKGLKPNAIY LFLVRAANAY GISDPSQISD
PVKTQDVLPT SQGVDHKQVQ RELGNAVLHL HNPTVLSSSS IEVHWTVDQQ
SQYIQGYKIL YRPSGANHGE SDWLVFEVRT PAKNSVVIPD LRKGVNYEIK
ARPFFNEFQG ADSEIKFAKT LEEAPSAPPQ GVTVSKNDGN GTAILVSWQP
PPEDTQNGMV QEYKVWCLGN ETRYHINKTV DGSTFSVVIP FLVPGIRYSV
EVAASTGAGS GVKSEPQFIQ LDAHGNPVSP EDQVSLAQQI SDVVKQPAFI
AGIGAACWII LMVFSIWLYR HRKKRNGLTS TYAGIRKVPS FTFTPTVTYQ
RGGEAVSSGG RPGLLNISEP AAQPWLADTW PNTGNNHNDC SISCCTAGNG
NSDSNLTTYS RPADCIANYN NQLDNKQTNL MLPESTVYGD VDLSNKINEM
KTFNSPNLKD GRFVNPSGQP TPYATTQLIQ SNLSNNMNNG SGDSGEKHWK
PLGQQKQEVA PVQYNIVEQN KLNKDYRAND TVPPTIPYNQ SYDQNTGGSY
NSSDRGSSTS GSQGHKKGAR TPKVPKQGGM NWADLLPPPP AHPPPHSNSE
EYNISVDESY DQEMPCPVPP ARMYLQQDEL EEEEDERGPT PPVRGAASSP
AAVSYSHQST ATLTPSPQEE LQPMLQDCPE ETGHMQHQPD RRRQPVSPPP
PPRPISPPHT YGYISGPLVS DMDTDAPEEE EDEADMEVAK MQTRRLLLRG
LEQTPASSVG DLESSVTGSM INGWGSASEE DNISSGRSSV SSSDGSFFTD
ADFAQAVAAA AEYAGLKVAR RQMQDAAGRR HFHASQCPRP TSPVSTDSNM
SAAVMQKTRP AKKLKHQPGH LRRETYTDDL PPPPVPPPAI KSPTAQSKTQ
LEVRPVVVPK LPSMDARTDR SSDRKGSSYK GREVLDGRQV VDMRTNPGDP
REAQEQQNDG KGRGNKAAKR DLPPAKTHLI QEDILPYCRP TFPTSNNPRD
PSSSSSMSSR GSGSRQREQA NVGRRNIAEM QVLGGYERGE DNNEELEETE
S
UCSC uc003dqc.3; human. [Q9Y6N7-1];

Category

Human ROBO1 ELISA Kit

Human deleted in U twenty twenty ELISA Kit;Human H-Robo-1 ELISA Kit;Human DUTT1 ELISA Kit;Human SAX3 ELISA Kit;Human roundabout guidance receptor 1 ELISA Kit;Human roundabout homolog 1 ELISA Kit;Human roundabout, axon guidance receptor, homolog 1 ELISA Kit;