Human MAT1A ELISA Kit

Alternative name
Human adoMet synthase 1 ELISA Kit;Human methionine adenosyltransferase 1 ELISA Kit;Human MAT 1 ELISA Kit;Human methionine adenosyltransferase I/III ELISA Kit;Human MAT-I/III ELISA Kit;Human AMS1 ELISA Kit;Human MATA1 ELISA Kit;Human MAT ELISA Kit;Human SAMS ELISA Kit;Human SAMS1 ELISA Kit;Human methionine adenosyltransferase 1A ELISA Kit;Human S-adenosylmethionine synthase isoform type-1 ELISA Kit;Human S-adenosylmethionine synthase ELISA Kit;Human S-adenosylmethionine synthetase isoform type-1 ELISA Kit;Human adoMet synthetase 1 ELISA Kit;Human methionine adenosyltransferase I, alpha ELISA Kit;
Catalog
E034051
species
Human
GeneMAT1A
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseHuman MAT1A ELISA Kit allows for the in vitro quantitative determination of MAT1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MAT1A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MAT1A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MAT1A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MAT1A can be calculated.
Human S-adenosylmethionine synthase isoform type-1 Protein information
Uniprot ID METK1_HUMAN
Uniprot AC Q00266; D3DWD5; Q5QP09;
UniGene Hs.282670;
GeneID 4143
KEGG hsa:4143;
Human S-adenosylmethionine synthase isoform type-1 Protein SEQUENCE
SEQUENCE 395 AA; 43648 MW; B3462A5670A5B0D4 CRC64;
MNGPVDGLCD HSLSEGVFMF TSESVGEGHP DKICDQISDA VLDAHLKQDP
NAKVACETVC KTGMVLLCGE ITSMAMVDYQ RVVRDTIKHI GYDDSAKGFD
FKTCNVLVAL EQQSPDIAQC VHLDRNEEDV GAGDQGLMFG YATDETEECM
PLTIILAHKL NARMADLRRS GLLPWLRPDS KTQVTVQYMQ DNGAVIPVRI
HTIVISVQHN EDITLEEMRR ALKEQVIRAV VPAKYLDEDT VYHLQPSGRF
VIGGPQGDAG VTGRKIIVDT YGGWGAHGGG AFSGKDYTKV DRSAAYAARW
VAKSLVKAGL CRRVLVQVSY AIGVAEPLSI SIFTYGTSQK TERELLDVVH
KNFDLRPGVI VRDLDLKKPI YQKTACYGHF GRSEFPWEVP RKLVF
UCSC uc001kbw.4; human.;

Category

Human MAT1A ELISA Kit