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Human S100A14 ELISA Kit

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  • Alternative name

    Human S100 calcium-binding protein A14 ELISA Kit;Human S114 ELISA Kit;Human S100A15 ELISA Kit;Human BCMP84 ELISA Kit;Human S100 calcium binding protein A14 ELISA Kit;Human protein S100-A14 ELISA Kit;Human breast cancer membrane protein 84 ELISA Kit;
  • Catalog
    E034082
  • species
    Human
  • GeneS100A14
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human S100A14. No significant cross-reactivity or interference between Human S100A14 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.4ng/ml
  • Intended UseHuman S100A14 ELISA Kit allows for the in vitro quantitative determination of S100A14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Introduction: S100A14 is a member of a subfamily of proteins related by Ca(2+)-binding motifs to the EF-hand Ca(2+)-binding protein superfamily. Expressed at highest levels in colon and at moderate levels in thymus, kidney, liver, small intestine, and lung. S100A14 is also overexpressed in ovary, breast and uterus tumours Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100A14. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for S100A14 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain S100A14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of S100A14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Protein S100-A14 Protein information
  • Uniprot ID S10AE_HUMAN
  • Uniprot AC Q9HCY8; Q5RHT0;
  • UniGene Hs.288998;
  • GeneID 57402
  • KEGG hsa:57402;
  • Human Protein S100-A14 Protein SEQUENCE

  • SEQUENCE 104 AA; 11662 MW; 97EF31A46B388E79 CRC64;

    MGQCRSANAE DAQEFSDVER AIETLIKNFH QYSVEGGKET LTPSELRDLV

    TQQLPHLMPS NCGLEEKIAN LGSCNDSKLE FRSFWELIGE AAKSVKLERP

    VRGH

  • UCSC uc001fce.4; human.;



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