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Human S100P ELISA Kit

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  • Alternative name

    Human migration-inducing gene 9 protein ELISA Kit;Human MIG9 ELISA Kit;Human protein S100-E ELISA Kit;Human S100 calcium-binding protein P ELISA Kit;Human S100E ELISA Kit;Human S100 calcium binding protein P ELISA Kit;Human protein S100-P ELISA Kit;
  • Catalog
    E034121
  • species
    Human
  • GeneS100P
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman S100P ELISA Kit allows for the in vitro quantitative determination of S100P , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Principle of the Assay: S100P ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for S100P. Standards or samples are then added to the microtiter plate wells and S100P if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of S100P present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for S100P are added to each well to "sandwich" the S100P immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain S100P and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100P concentration in each sample is interpolated from this standard curve.
  • Human Protein S100-P Protein information
  • Uniprot ID S100P_HUMAN
  • Uniprot AC P25815; Q5J7W2;
  • UniGene Hs.2962;
  • GeneID 6286
  • KEGG hsa:6286;
  • Human Protein S100-P Protein SEQUENCE

  • SEQUENCE 95 AA; 10400 MW; 786E6E3F3EACC6C1 CRC64;

    MTELETAMGM IIDVFSRYSG SEGSTQTLTK GELKVLMEKE LPGFLQSGKD

    KDAVDKLLKD LDANGDAQVD FSEFIVFVAA ITSACHKYFE KAGLK

  • UCSC uc003gjl.4; human.;



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