Human SCUBE1 ELISA Kit

Alternative name
Human signal peptide, CUB domain and EGF like domain containing 1 ELISA Kit;Human signal peptide, CUB and EGF-like domain-containing protein 1 ELISA Kit;Human signal peptide, CUB domain, EGF-like 1 ELISA Kit;Human signal peptide-CUB domain-EGF-related 1 ELISA Kit;
Catalog
E034363
species
Human
GeneSCUBE1
SpecificityThis kit recognizes natural and recombinant Human SCUBE1. No significant cross-reactivity or interference between Human SCUBE1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of Human SCUBE1 is 0.375ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
Intended UseHuman SCUBE1 ELISA Kit allows for the in vitro quantitative determination of SCUBE1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SCUBE1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SCUBE1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SCUBE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SCUBE1. You can calculate the concentration of SCUBE1 in the samples by comparing the OD of the samples to the standard curve.
Human Signal peptide, CUB and EGF-like domain-containing protein 1 Protein information
Uniprot ID SCUB1_HUMAN
Uniprot AC Q8IWY4; Q5R336;
UniGene Hs.133995;
GeneID 80274
KEGG hsa:80274;
Human Signal peptide, CUB and EGF-like domain-containing protein 1 Protein SEQUENCE
SEQUENCE 988 AA; 107910 MW; AB2C2AB53F3D3C2B CRC64;
MGAAAVRWHL CVLLALGTRG RLAGGSGLPG SVDVDECSEG TDDCHIDAIC
QNTPKSYKCL CKPGYKGEGK QCEDIDECEN DYYNGGCVHE CINIPGNYRC
TCFDGFMLAH DGHNCLDVDE CQDNNGGCQQ ICVNAMGSYE CQCHSGFFLS
DNQHTCIHRS NEGMNCMNKD HGCAHICRET PKGGVACDCR PGFDLAQNQK
DCTLTCNYGN GGCQHSCEDT DTGPTCGCHQ KYALHSDGRT CIETCAVNNG
GCDRTCKDTA TGVRCSCPVG FTLQPDGKTC KDINECLVNN GGCDHFCRNT
VGSFECGCRK GYKLLTDERT CQDIDECSFE RTCDHICINS PGSFQCLCHR
GYILYGTTHC GDVDECSMSN GSCDQGCVNT KGSYECVCPP GRRLHWNGKD
CVETGKCLSR AKTSPRAQLS CSKAGGVESC FLSCPAHTLF VPDSENSYVL
SCGVPGPQGK ALQKRNGTSS GLGPSCSDAP TTPIKQKARF KIRDAKCHLR
PHSQARAKET ARQPLLDHCH VTFVTLKCDS SKKRRRGRKS PSKEVSHITA
EFEIETKMEE ASDTCEADCL RKRAEQSLQA AIKTLRKSIG RQQFYVQVSG
TEYEVAQRPA KALEGQGACG AGQVLQDSKC VACGPGTHFG GELGQCVSCM
PGTYQDMEGQ LSCTPCPSSD GLGLPGARNV SECGGQCSPG FFSADGFKPC
QACPVGTYQP EPGRTGCFPC GGGLLTKHEG TTSFQDCEAK VHCSPGHHYN
TTTHRCIRCP VGTYQPEFGQ NHCITCPGNT STDFDGSTNV THCKNQHCGG
ELGDYTGYIE SPNYPGDYPA NAECVWHIAP PPKRRILIVV PEIFLPIEDE
CGDVLVMRKS ASPTSITTYE TCQTYERPIA FTSRSRKLWI QFKSNEGNSG
KGFQVPYVTY DEDYQQLIED IVRDGRLYAS ENHQEILKDK KLIKALFDVL
AHPQNYFKYT AQESKEMFPR SFIKLLRSKV SRFLRPYK
UCSC uc003bdt.3; human.;

Category

Human SCUBE1 ELISA Kit

Human signal peptide, CUB domain and EGF like domain containing 1 ELISA Kit;Human signal peptide, CUB and EGF-like domain-containing protein 1 ELISA Kit;Human signal peptide, CUB domain, EGF-like 1 ELISA Kit;Human signal peptide-CUB domain-EGF-related 1 ELISA Kit;