Human SCGN ELISA Kit

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  • Alternative name

    Human SECRET ELISA Kit;Human CALBL ELISA Kit;Human DJ501N12.8 ELISA Kit;Human SEGN ELISA Kit;Human setagin ELISA Kit;Human secretagogin, EF-hand calcium binding protein ELISA Kit;Human secretagogin ELISA Kit;

  • Catalog
    E034418
  • species
    Human
  • GeneSCGN
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Secretagogin (SCGN). No significant cross-reactivity or interference between Secretagogin (SCGN) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 12.7pg/mL.
  • Intended UseHuman SCGN ELISA Kit allows for the in vitro quantitative determination of SCGN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretagogin (SCGN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secretagogin (SCGN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secretagogin (SCGN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secretagogin (SCGN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Secretagogin Protein information
  • Uniprot ID SEGN_HUMAN
  • Uniprot AC O76038; A8K0B2; Q5VV44; Q96QV7; Q9UJF6;
  • UniGene Hs.116428;
  • GeneID 10590
  • KEGG hsa:10590;
  • Human Secretagogin Protein SEQUENCE
  • SEQUENCE 276 AA; 32040 MW; EB970BF7B8763F33 CRC64;

    MDSSREPTLG RLDAAGFWQV WQRFDADEKG YIEEKELDAF FLHMLMKLGT

    DDTVMKANLH KVKQQFMTTQ DASKDGRIRM KELAGMFLSE DENFLLLFRR

    ENPLDSSVEF MQIWRKYDAD SSGFISAAEL RNFLRDLFLH HKKAISEAKL

    EEYTGTMMKI FDRNKDGRLD LNDLARILAL QENFLLQFKM DACSTEERKR

    DFEKIFAYYD VSKTGALEGP EVDGFVKDMM ELVQPSISGV DLDKFREILL

    RHCDVNKDGK IQKSELALCL GLKINP

  • UCSC uc003nfb.4; human.;



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