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Human SFRP1 ELISA Kit

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  • Alternative name

    Human FRP-1 ELISA Kit;Human sFRP-1 ELISA Kit;Human secreted apoptosis-related protein 2 ELISA Kit;Human SARP-2 ELISA Kit;Human FRP ELISA Kit;Human FRP1 ELISA Kit;Human SARP2 ELISA Kit;Human FrzA ELISA Kit;Human secreted frizzled related protein 1 ELISA Kit;Human secreted frizzled-related protein 1 ELISA Kit;Human frizzled-related protein ELISA Kit;
  • Catalog
    E034420
  • species
    Human
  • GeneSFRP1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SFRP1. No significant cross-reactivity or interference between SFRP1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SFRP1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman SFRP1 ELISA Kit allows for the in vitro quantitative determination of SFRP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical    Principle of the assay: SFRP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SFRP1 antibody and an SFRP1-HRP conjugate. The assay sample and buffer are incubated together with SFRP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SFRP1 concentration since SFRP1 from samples and SFRP1-HRP conjugate compete for the anti-SFRP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SFRP1 from the sample, fewer sites are left to bind SFRP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SFRP1 concentration in each sample is interpolated from this standard curve.
  • Human Secreted frizzled-related protein 1 Protein information
  • Uniprot ID SFRP1_HUMAN
  • Uniprot AC Q8N474; O00546; O14779;
  • UniGene Hs.213424;
  • GeneID 6422
  • KEGG hsa:6422;
  • Human Secreted frizzled-related protein 1 Protein SEQUENCE

  • SEQUENCE 314 AA; 35386 MW; 29DD948706EB7143 CRC64;

    MGIGRSEGGR RGAALGVLLA LGAALLAVGS ASEYDYVSFQ SDIGPYQSGR

    FYTKPPQCVD IPADLRLCHN VGYKKMVLPN LLEHETMAEV KQQASSWVPL

    LNKNCHAGTQ VFLCSLFAPV CLDRPIYPCR WLCEAVRDSC EPVMQFFGFY

    WPEMLKCDKF PEGDVCIAMT PPNATEASKP QGTTVCPPCD NELKSEAIIE

    HLCASEFALR MKIKEVKKEN GDKKIVPKKK KPLKLGPIKK KDLKKLVLYL

    KNGADCPCHQ LDNLSHHFLI MGRKVKSQYL LTAIHKWDKK NKEFKNFMKK

    MKNHECPTFQ SVFK

  • UCSC uc003xnt.4; human.;



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