Human SFRP2 ELISA Kit

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  • Alternative name

    Human FRP-2 ELISA Kit;Human sFRP-2 ELISA Kit;Human Secreted apoptosis-related protein 1 ELISA Kit;Human SARP-1 ELISA Kit;Human FRP2 ELISA Kit;Human SARP1 ELISA Kit;Human SDF-5 ELISA Kit;Human secreted frizzled related protein 2 ELISA Kit;Human secreted frizzled-related protein 2 ELISA Kit;Human secreted apoptosis related protein 1 ELISA Kit;Human testicular tissue protein Li 170 ELISA Kit;

  • Catalog
    E034425
  • species
    Human
  • GeneSFRP2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Secreted Frizzled Related Protein 2 (SFRP2). No significant cross-reactivity or interference between Secreted Frizzled Related Protein 2 (SFRP2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.53ng/mL.
  • Intended UseHuman SFRP2 ELISA Kit allows for the in vitro quantitative determination of SFRP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 2 (SFRP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 2 (SFRP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Frizzled Related Protein 2 (SFRP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secreted Frizzled Related Protein 2 (SFRP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Secreted frizzled-related protein 2 Protein information
  • Uniprot ID SFRP2_HUMAN
  • Uniprot AC Q96HF1; B3KQR2; O14778; Q9HAP5;
  • UniGene Hs.481022;
  • GeneID 6423
  • KEGG hsa:6423;
  • Human Secreted frizzled-related protein 2 Protein SEQUENCE
  • SEQUENCE 295 AA; 33490 MW; 97597971541BFBC4 CRC64;

    MLQGPGSLLL LFLASHCCLG SARGLFLFGQ PDFSYKRSNC KPIPANLQLC

    HGIEYQNMRL PNLLGHETMK EVLEQAGAWI PLVMKQCHPD TKKFLCSLFA

    PVCLDDLDET IQPCHSLCVQ VKDRCAPVMS AFGFPWPDML ECDRFPQDND

    LCIPLASSDH LLPATEEAPK VCEACKNKND DDNDIMETLC KNDFALKIKV

    KEITYINRDT KIILETKSKT IYKLNGVSER DLKKSVLWLK DSLQCTCEEM

    NDINAPYLVM GQKQGGELVI TSVKRWQKGQ REFKRISRSI RKLQC

  • UCSC uc003inv.2; human.;



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