Human SPR ELISA Kit

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  • Alternative name

    Human SPR ELISA Kit;Human SDR38C1 ELISA Kit;Human sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase) ELISA Kit;Human sepiapterin reductase ELISA Kit;Human short chain dehydrogenase/reductase family 38C, member 1 ELISA Kit;

  • Catalog
    E034652
  • species
    Human
  • GeneSPR
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Sepiapterin Reductase (SPR). No significant cross-reactivity or interference between Sepiapterin Reductase (SPR) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.108ng/mL.
  • Intended UseHuman SPR ELISA Kit allows for the in vitro quantitative determination of SPR , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sepiapterin Reductase (SPR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sepiapterin Reductase (SPR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sepiapterin Reductase (SPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sepiapterin Reductase (SPR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Sepiapterin reductase Protein information
  • Uniprot ID SPRE_HUMAN
  • Uniprot AC P35270; A8K741; D6W5H2; Q53GI9; Q9UBB1;
  • UniGene Hs.301540;
  • GeneID 6697
  • KEGG hsa:6697;
  • Human Sepiapterin reductase Protein SEQUENCE
  • SEQUENCE 261 AA; 28048 MW; 9C9BF76212826F47 CRC64;

    MEGGLGRAVC LLTGASRGFG RTLAPLLASL LSPGSVLVLS ARNDEALRQL

    EAELGAERSG LRVVRVPADL GAEAGLQQLL GALRELPRPK GLQRLLLINN

    AGSLGDVSKG FVDLSDSTQV NNYWALNLTS MLCLTSSVLK AFPDSPGLNR

    TVVNISSLCA LQPFKGWALY CAGKAARDML FQVLALEEPN VRVLNYAPGP

    LDTDMQQLAR ETSVDPDMRK GLQELKAKGK LVDCKVSAQK LLSLLEKDEF

    KSGAHVDFYD K

  • UCSC uc002sik.3; human.;



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