Human SPINT1 ELISA Kit

Alternative name
Human hepatocyte growth factor activator inhibitor type 1 ELISA Kit;Human HAI-1 ELISA Kit;Human HAI1 ELISA Kit;Human HAI ELISA Kit;Human MANSC2 ELISA Kit;Human serine peptidase inhibitor, Kunitz type 1 ELISA Kit;Human kunitz-type protease inhibitor 1 ELISA Kit;Human serine protease inhibitor, Kunitz type 1 ELISA Kit;
Catalog
E034730
species
Human
GeneSPINT1
SpecificityThis assay has high sensitivity and excellent specificity for detection of human SPINT1. No significant cross-reactivity or interference between human SPINT1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human SPINT1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
Intended UseHuman SPINT1 ELISA Kit allows for the in vitro quantitative determination of SPINT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPriciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPINT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPINT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPINT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPINT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Kunitz-type protease inhibitor 1 Protein information
Uniprot ID SPIT1_HUMAN
Uniprot AC O43278; Q7Z7D2;
UniGene Hs.233950;
GeneID 6692
KEGG hsa:6692;
Human Kunitz-type protease inhibitor 1 Protein SEQUENCE
SEQUENCE 529 AA; 58398 MW; A87F286C23C73422 CRC64;
MAPARTMARA RLAPAGIPAV ALWLLCTLGL QGTQAGPPPA PPGLPAGADC
LNSFTAGVPG FVLDTNASVS NGATFLESPT VRRGWDCVRA CCTTQNCNLA
LVELQPDRGE DAIAACFLIN CLYEQNFVCK FAPREGFINY LTREVYRSYR
QLRTQGFGGS GIPKAWAGID LKVQPQEPLV LKDVENTDWR LLRGDTDVRV
ERKDPNQVEL WGLKEGTYLF QLTVTSSDHP EDTANVTVTV LSTKQTEDYC
LASNKVGRCR GSFPRWYYDP TEQICKSFVY GGCLGNKNNY LREEECILAC
RGVQGGPLRG SSGAQATFPQ GPSMERRHPV CSGTCQPTQF RCSNGCCIDS
FLECDDTPNC PDASDEAACE KYTSGFDELQ RIHFPSDKGH CVDLPDTGLC
KESIPRWYYN PFSEHCARFT YGGCYGNKNN FEEEQQCLES CRGISKKDVF
GLRREIPIPS TGSVEMAVAV FLVICIVVVV AILGYCFFKN QRKDFHGHHH
HPPPTPASST VSTTEDTEHL VYNHTTRPL
UCSC uc001zna.4; human. [O43278-1];

Category

Human SPINT1 ELISA Kit