Human SIK2 ELISA Kit

Alternative name
Human qin-induced kinase ELISA Kit;Human Salt-inducible kinase 2 ELISA Kit;Human SIK-2 ELISA Kit;Human serine/threonine-protein kinase SNF1-like kinase 2 ELISA Kit;Human LOH11CR1I ELISA Kit;Human QIK ELISA Kit;Human SNF1LK2 ELISA Kit;Human salt inducible kinase 2 ELISA Kit;Human serine/threonine-protein kinase SIK2 ELISA Kit;Human SNF1-like kinase 2 ELISA Kit;Human salt-inducible protein kinase 2 ELISA Kit;Human salt-inducible serine/threonine kinase 2 ELISA Kit;
Catalog
E034795
species
Human
GeneSIK2
SpecificityThis assay has high sensitivity and excellent specificity for detection of SIK2. No significant cross-reactivity or interference between SIK2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SIK2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman SIK2 ELISA Kit allows for the in vitro quantitative determination of SIK2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: SIK2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SIK2 antibody and an SIK2-HRP conjugate. The assay sample and buffer are incubated together with SIK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SIK2 concentration since SIK2 from samples and SIK2-HRP conjugate compete for the anti-SIK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SIK2 from the sample, fewer sites are left to bind SIK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SIK2 concentration in each sample is interpolated from this standard curve.
Human Serine/threonine-protein kinase SIK2 Protein information
Uniprot ID SIK2_HUMAN
Uniprot AC Q96CZ8;
UniGene Hs.269128;
GeneID 23235
KEGG hsa:23235;
Human Serine/threonine-protein kinase SIK2 Protein SEQUENCE
SEQUENCE 926 AA; 103915 MW; 9D7F9BF81FD65CCF CRC64;
MVMADGPRHL QRGPVRVGFY DIEGTLGKGN FAVVKLGRHR ITKTEVAIKI
IDKSQLDAVN LEKIYREVQI MKMLDHPHII KLYQVMETKS MLYLVTEYAK
NGEIFDYLAN HGRLNESEAR RKFWQILSAV DYCHGRKIVH RDLKAENLLL
DNNMNIKIAD FGFGNFFKSG ELLATWCGSP PYAAPEVFEG QQYEGPQLDI
WSMGVVLYVL VCGALPFDGP TLPILRQRVL EGRFRIPYFM SEDCEHLIRR
MLVLDPSKRL TIAQIKEHKW MLIEVPVQRP VLYPQEQENE PSIGEFNEQV
LRLMHSLGID QQKTIESLQN KSYNHFAAIY FLLVERLKSH RSSFPVEQRL
DGRQRRPSTI AEQTVAKAQT VGLPVTMHSP NMRLLRSALL PQASNVEAFS
FPASGCQAEA AFMEEECVDT PKVNGCLLDP VPPVLVRKGC QSLPSNMMET
SIDEGLETEG EAEEDPAHAF EAFQSTRSGQ RRHTLSEVTN QLVVMPGAGK
IFSMNDSPSL DSVDSEYDMG SVQRDLNFLE DNPSLKDIML ANQPSPRMTS
PFISLRPTNP AMQALSSQKR EVHNRSPVSF REGRRASDTS LTQGIVAFRQ
HLQNLARTKG ILELNKVQLL YEQIGPEADP NLAPAAPQLQ DLASSCPQEE
VSQQQESVST LPASVHPQLS PRQSLETQYL QHRLQKPSLL SKAQNTCQLY
CKEPPRSLEQ QLQEHRLQQK RLFLQKQSQL QAYFNQMQIA ESSYPQPSQQ
LPLPRQETPP PSQQAPPFSL TQPLSPVLEP SSEQMQYSPF LSQYQEMQLQ
PLPSTSGPRA APPLPTQLQQ QQPPPPPPPP PPRQPGAAPA PLQFSYQTCE
LPSAASPAPD YPTPCQYPVD GAQQSDLTGP DCPRSPGLQE APSSYDPLAL
SELPGLFDCE MLDAVDPQHN GYVLVN
UCSC uc001plt.4; human.;

Category

Human SIK2 ELISA Kit