Human SERPINB4 ELISA Kit

Alternative name
Human LEUPIN ELISA Kit;Human peptidase inhibitor 11 ELISA Kit;Human PI-11 ELISA Kit;Human squamous cell carcinoma antigen 2 ELISA Kit;Human SCCA-2 ELISA Kit;Human PI11 ELISA Kit;Human SCCA2 ELISA Kit;Human SCCA1 ELISA Kit;Human serpin family B member 4 ELISA Kit;Human serpin B4 ELISA Kit;Human SCCA2/SCCA1 fusion protein ELISA Kit;Human protease inhibitor (leucine-serpin) ELISA Kit;Human serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 4 ELISA Kit;Human serpin peptidase inhibitor, clade B (ovalbumin), member 4 ELISA Kit;Human squamous cell carcinoma antigen 1 ELISA Kit;
Catalog
E034870
species
Human
GeneSERPINB4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman SERPINB4 ELISA Kit allows for the in vitro quantitative determination of SERPINB4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- SERPINB4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- SERPINB4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SERPINB4 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SERPINB4 can be calculated.
Human Serpin B4 Protein information
Uniprot ID SPB4_HUMAN
Uniprot AC P48594; A8K847;
UniGene Hs.123035;
GeneID 6318
KEGG hsa:6318;
Human Serpin B4 Protein SEQUENCE
SEQUENCE 390 AA; 44854 MW; 04E213CD892587D5 CRC64;
MNSLSEANTK FMFDLFQQFR KSKENNIFYS PISITSALGM VLLGAKDNTA
QQISKVLHFD QVTENTTEKA ATYHVDRSGN VHHQFQKLLT EFNKSTDAYE
LKIANKLFGE KTYQFLQEYL DAIKKFYQTS VESTDFANAP EESRKKINSW
VESQTNEKIK NLFPDGTIGN DTTLVLVNAI YFKGQWENKF KKENTKEEKF
WPNKNTYKSV QMMRQYNSFN FALLEDVQAK VLEIPYKGKD LSMIVLLPNE
IDGLQKLEEK LTAEKLMEWT SLQNMRETCV DLHLPRFKME ESYDLKDTLR
TMGMVNIFNG DADLSGMTWS HGLSVSKVLH KAFVEVTEEG VEAAAATAVV
VVELSSPSTN EEFCCNHPFL FFIRQNKTNS ILFYGRFSSP
UCSC uc002ljf.4; human.;

Category

Human SERPINB4 ELISA Kit