Human SIPA1 ELISA Kit

Alternative name
Human sipa-1 ELISA Kit;Human GTPase-activating protein Spa-1 ELISA Kit;Human p130 SPA-1 ELISA Kit;Human SPA1 ELISA Kit;Human signal-induced proliferation-associated 1 ELISA Kit;Human signal-induced proliferation-associated protein 1 ELISA Kit;Human signal-induced proliferation-associated gene 1 ELISA Kit;
Catalog
E035300
species
Human
GeneSIPA1
SpecificityThis assay has high sensitivity and excellent specificity for detection of human SIPA1. No significant cross-reactivity or interference between human SIPA1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human SIPA1 is typically less than 6.25 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman SIPA1 ELISA Kit allows for the in vitro quantitative determination of SIPA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SIPA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIPA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIPA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIPA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Signal-induced proliferation-associated protein 1 Protein information
Uniprot ID SIPA1_HUMAN
Uniprot AC Q96FS4; O14518; O60484; O60618; Q2YD83;
UniGene Hs.530477;
GeneID 6494
KEGG hsa:6494;
Human Signal-induced proliferation-associated protein 1 Protein SEQUENCE
SEQUENCE 1042 AA; 112149 MW; FB52C7231EA3B6B9 CRC64;
MPMWAGGVGS PRRGMAPAST DDLFARKLRQ PARPPLTPHT FEPRPVRGPL
LRSGSDAGEA RPPTPASPRA RAHSHEEASR PAATSTRLFT DPLALLGLPA
EEPEPAFPPV LEPRWFAHYD VQSLLFDWAP RSQGMGSHSE ASSGTLASAE
DQAASSDLLH GAPGFVCELG GEGELGLGGP ASPPVPPALP NAAVSILEEP
QNRTSAYSLE HADLGAGYYR KYFYGKEHQN FFGMDESLGP VAVSLRREEK
EGSGGGTLHS YRVIVRTTQL RTLRGTISED ALPPGPPRGL SPRKLLEHVA
PQLSPSCLRL GSASPKVPRT LLTLDEQVLS FQRKVGILYC RAGQGSEEEM
YNNQEAGPAF MQFLTLLGDV VRLKGFESYR AQLDTKTDST GTHSLYTTYQ
DHEIMFHVST MLPYTPNNQQ QLLRKRHIGN DIVTIVFQEP GSKPFCPTTI
RSHFQHVFLV VRAHTPCTPH TTYRVAVSRT QDTPAFGPAL PAGGGPFAAN
ADFRAFLLAK ALNGEQAAGH ARQFHAMATR TRQQYLQDLA TNEVTTTSLD
SASRFGLPSL GGRRRAAPRG PGAELQAAGS LVWGVRAAPG ARVAAGAQAS
GPEGIEVPCL LGISAEALVL VAPRDGRVVF NCACRDVLAW TFSEQQLDLY
HGRGEAITLR FDGSPGQAVG EVVARLQLVS RGCETRELAL PRDGQGRLGF
EVDAEGFVTH VERFTFAETA GLRPGARLLR VCGQTLPSLR PEAAAQLLRS
APKVCVTVLP PDESGRPRRS FSELYTLSLQ EPSRRGAPDP VQDEVQGVTL
LPTTKQLLHL CLQDGGSPPG PGDLAEERTE FLHSQNSLSP RSSLSDEAPV
LPNTTPDLLL ATTAKPSVPS ADSETPLTQD RPGSPSGSED KGNPAPELRA
SFLPRTLSLR NSISRIMSEA GSGTLEDEWQ AISEIASTCN TILESLSREG
QPIPESGDPK GTPKSDAEPE PGNLSEKVSH LESMLRKLQE DLQKEKADRA
ALEEEVRSLR HNNRRLQAES ESAATRLLLA SKQLGSPTAD LA
UCSC uc001ofb.3; human.;

Category

Human SIPA1 ELISA Kit