Human SIRT2 ELISA Kit

Alternative name
Human regulatory protein SIR2 homolog 2 ELISA Kit;Human SIR2-like protein 2 ELISA Kit;Human SIR2L ELISA Kit;Human SIR2L2 ELISA Kit;Human SIR2 ELISA Kit;Human sirtuin 2 ELISA Kit;Human NAD-dependent protein deacetylase sirtuin-2 ELISA Kit;Human NAD-dependent deacetylase sirtuin-2 ELISA Kit;Human silent information regulator 2 ELISA Kit;Human sir2-related protein type 2 ELISA Kit;Human sirtuin type 2 ELISA Kit;
Catalog
E035345
species
Human
GeneSIRT2
SpecificityThis assay has high sensitivity and excellent specificity for detection of human SIRT2. No significant cross-reactivity or interference between human SIRT2 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human SIRT2 is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman SIRT2 ELISA Kit allows for the in vitro quantitative determination of SIRT2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SIRT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIRT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRT2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human NAD-dependent protein deacetylase sirtuin-2 Protein information
Uniprot ID SIR2_HUMAN
Uniprot AC U5TP13;
UniGene Hs.466693;
GeneID 22933
KEGG hsa:22933;
Human NAD-dependent protein deacetylase sirtuin-2 Protein SEQUENCE
SEQUENCE 389 AA; 43182 MW; A392442A8F6316F1 CRC64;
MAEPDPSHPL ETQAGKVQEA QDSDSDSEGG AAGGEADMDF LRNLFSQTLS
LGSQKERLLD ELTLEGVARY MQSERCRRVI CLVGAGISTS AGIPDFRSPS
TGLYDNLEKY HLPYPEAIFE ISYFKKHPEP FFALAKELYP GQFKPTICHY
FMRLLKDKGL LLRCYTQNID TLERIAGLEQ EDLVEAHGTF YTSHCVSASC
RHEYPLSWMK EKIFSEVTPK CEDCQSLVKP DIVFFGESLP ARFFSCMQSD
FLKVDLLLVM GTSLQVQPFA SLISKAPLST PRLLINKEKA GQSDPFLGMI
MGLGGGMDFD SKKAYRDVAW LGECDQGCLA LAELLGWKKE LEDLVRREHA
SIDAQSGAGV PNPSTSASPK KSPPPAKDEA RTTEREKPQ
UCSC uc002ojt.3; human. [Q8IXJ6-1];

Category

Human SIRT2 ELISA Kit