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Alternative name
NAD-dependent protein deacetylase sirtuin-4 ELISA KIT; NAD-dependent ADP-ribosyltransferase sirtuin-4 (EC:2.4.2.-) ELISA KIT; Regulatory protein SIR2 homolog 4 ELISA KIT; SIR2-like protein 4SIRT4 ELISA KIT; SIR2L4 ELISA KIT
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Catalog
E035347
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species
Human
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GeneSIRT4
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Human SIRT4. No significant cross-reactivity or interference between Human SIRT4 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseHuman SIRT4 ELISA Kit allows for the in vitro quantitative determination of SIRT4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SIRT4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIRT4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRT4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRT4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
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