Human SOCS3 ELISA Kit

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  • Alternative name

    Human SOCS-3 ELISA Kit;Human cytokine-inducible SH2 protein 3 ELISA Kit;Human CIS-3 ELISA Kit;Human STAT-induced STAT inhibitor 3 ELISA Kit;Human SSI-3 ELISA Kit;Human CIS3 ELISA Kit;Human SSI3 ELISA Kit;Human ATOD4 ELISA Kit;Human Cish3 ELISA Kit;Human suppressor of cytokine signaling 3 ELISA Kit;

  • Catalog
    E035578
  • species
    Human
  • GeneSOCS3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human SOCS3. No significant cross-reactivity or interference between Human SOCS3 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human SOCS3 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity9.375pg/mL
  • Intended UseHuman SOCS3 ELISA Kit allows for the in vitro quantitative determination of SOCS3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SOCS3 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SOCS3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human SOCS3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SOCS3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human SOCS3. You can calculate the concentration of Human SOCS3 in the samples by comparing the OD of the samples to the standard curve.
  • Human Suppressor of cytokine signaling 3 Protein information
  • Uniprot ID SOCS3_HUMAN
  • Uniprot AC O14543; O14509;
  • UniGene Hs.527973; Hs.703620;
  • GeneID 9021
  • KEGG hsa:9021;
  • Human Suppressor of cytokine signaling 3 Protein SEQUENCE
  • SEQUENCE 225 AA; 24770 MW; 08581DC411EFFF19 CRC64;

    MVTHSKFPAA GMSRPLDTSL RLKTFSSKSE YQLVVNAVRK LQESGFYWSA

    VTGGEANLLL SAEPAGTFLI RDSSDQRHFF TLSVKTQSGT KNLRIQCEGG

    SFSLQSDPRS TQPVPRFDCV LKLVHHYMPP PGAPSFPSPP TEPSSEVPEQ

    PSAQPLPGSP PRRAYYIYSG GEKIPLVLSR PLSSNVATLQ HLCRKTVNGH

    LDSYEKVTQL PGPIREFLDQ YDAPL




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