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Human ATP1A2 ELISA Kit

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  • Alternative name

    Human Na(+)/K(+) ATPase alpha-2 subunit ELISA Kit;Human sodium pump subunit alpha-2 ELISA Kit;Human KIAA0778 ELISA Kit;Human FHM2 ELISA Kit;Human MHP2 ELISA Kit;Human ATPase Na+/K+ transporting subunit alpha 2 ELISA Kit;Human sodium/potassium-transporting ATPase subunit alpha-2 ELISA Kit;Human ATPase Na+/K+ transporting alpha 2 polypeptide ELISA Kit;Human Na+/K+ ATPase, alpha-A(+) catalytic polypeptide ELISA Kit;Human Na+/K+ ATPase, alpha-B polypeptide ELISA Kit;Human sodium-potassium ATPase catalytic subunit alpha-2 ELISA Kit;Human sodium/potassium-transporting ATPase alpha-2 chain ELISA Kit;
  • Catalog
    E035627
  • species
    Human
  • GeneATP1A2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ATP1A2. No significant cross-reactivity or interference between ATP1A2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATP1A2 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ATP1A2 ELISA Kit allows for the in vitro quantitative determination of ATP1A2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeuroscience
  • Product Description
    specifical    Intended Uses: This ATP1A2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATP1A2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ATP1A2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ATP1A2 antibody and an ATP1A2-HRP conjugate. The assay sample and buffer are incubated together with ATP1A2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP1A2 concentration since ATP1A2 from samples and ATP1A2-HRP conjugate compete for the anti-ATP1A2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP1A2 from the sample, fewer sites are left to bind ATP1A2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP1A2 concentration in each sample is interpolated from this standard curve.
  • Human Sodium/potassium-transporting ATPase subunit alpha-2 Protein information
  • Uniprot ID AT1A2_HUMAN
  • Uniprot AC P50993; D3DVE4; Q07059; Q5JW74; Q86UZ5; Q9UQ25;
  • UniGene Hs.34114;
  • GeneID 477
  • KEGG hsa:477;
  • Human Sodium/potassium-transporting ATPase subunit alpha-2 Protein SEQUENCE

  • SEQUENCE 1020 AA; 112265 MW; AFBD8EA94FFB4FC3 CRC64;

    MGRGAGREYS PAATTAENGG GKKKQKEKEL DELKKEVAMD DHKLSLDELG

    RKYQVDLSKG LTNQRAQDVL ARDGPNALTP PPTTPEWVKF CRQLFGGFSI

    LLWIGAILCF LAYGIQAAME DEPSNDNLYL GVVLAAVVIV TGCFSYYQEA

    KSSKIMDSFK NMVPQQALVI REGEKMQINA EEVVVGDLVE VKGGDRVPAD

    LRIISSHGCK VDNSSLTGES EPQTRSPEFT HENPLETRNI CFFSTNCVEG

    TARGIVIATG DRTVMGRIAT LASGLEVGRT PIAMEIEHFI QLITGVAVFL

    GVSFFVLSLI LGYSWLEAVI FLIGIIVANV PEGLLATVTV CLTLTAKRMA

    RKNCLVKNLE AVETLGSTST ICSDKTGTLT QNRMTVAHMW FDNQIHEADT

    TEDQSGATFD KRSPTWTALS RIAGLCNRAV FKAGQENISV SKRDTAGDAS

    ESALLKCIEL SCGSVRKMRD RNPKVAEIPF NSTNKYQLSI HEREDSPQSH

    VLVMKGAPER ILDRCSTILV QGKEIPLDKE MQDAFQNAYM ELGGLGERVL

    GFCQLNLPSG KFPRGFKFDT DELNFPTEKL CFVGLMSMID PPRAAVPDAV

    GKCRSAGIKV IMVTGDHPIT AKAIAKGVGI ISEGNETVED IAARLNIPMS

    QVNPREAKAC VVHGSDLKDM TSEQLDEILK NHTEIVFART SPQQKLIIVE

    GCQRQGAIVA VTGDGVNDSP ALKKADIGIA MGISGSDVSK QAADMILLDD

    NFASIVTGVE EGRLIFDNLK KSIAYTLTSN IPEITPFLLF IIANIPLPLG

    TVTILCIDLG TDMVPAISLA YEAAESDIMK RQPRNSQTDK LVNERLISMA

    YGQIGMIQAL GGFFTYFVIL AENGFLPSRL LGIRLDWDDR TMNDLEDSYG

    QEWTYEQRKV VEFTCHTAFF ASIVVVQWAD LIICKTRRNS VFQQGMKNKI

    LIFGLLEETA LAAFLSYCPG MGVALRMYPL KVTWWFCAFP YSLLIFIYDE

    VRKLILRRYP GGWVEKETYY

  • UCSC uc001fvc.4; human.;



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