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Human ATP1A3 ELISA Kit

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  • Alternative name

    Human Na(+)/K(+) ATPase alpha-3 subunit ELISA Kit;Human Na(+)/K(+) ATPase alpha(III) subunit ELISA Kit;Human sodium pump subunit alpha-3 ELISA Kit;Human AHC2 ELISA Kit;Human ATP1A1 ELISA Kit;Human CAPOS ELISA Kit;Human DYT12 ELISA Kit;Human RDP ELISA Kit;Human ATPase Na+/K+ transporting subunit alpha 3 ELISA Kit;Human sodium/potassium-transporting ATPase subunit alpha-3 ELISA Kit;Human ATPase, Na+/K+ transporting, alpha 3 polypeptide ELISA Kit;Human Na+, K+ activated adenosine triphosphatase alpha subunit ELISA Kit;Human Na+/K+ ATPase 3 ELISA Kit;Human sodium-potassium ATPase catalytic subunit alpha-3 ELISA Kit;Human sodium-potassium-ATPase, alpha 3 polypeptide ELISA Kit;Human sodium/potassium-transporting ATPase alpha-3 chain ELISA Kit;
  • Catalog
    E035628
  • species
    Human
  • GeneATP1A3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ATP1A3. No significant cross-reactivity or interference between ATP1A3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATP1A3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ATP1A3 ELISA Kit allows for the in vitro quantitative determination of ATP1A3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeuroscience
  • Product Description
    specifical    Intended Uses: This ATP1A3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATP1A3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ATP1A3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ATP1A3 antibody and an ATP1A3-HRP conjugate. The assay sample and buffer are incubated together with ATP1A3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP1A3 concentration since ATP1A3 from samples and ATP1A3-HRP conjugate compete for the anti-ATP1A3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP1A3 from the sample, fewer sites are left to bind ATP1A3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP1A3 concentration in each sample is interpolated from this standard curve.
  • Human Sodium/potassium-transporting ATPase subunit alpha-3 Protein information
  • Uniprot ID AT1A3_HUMAN
  • Uniprot AC P13637; B7Z2T0; B7Z401; F5H6J6; Q16732; Q16735; Q969K5;
  • UniGene Hs.515427;
  • GeneID 478
  • KEGG hsa:478;
  • Human Sodium/potassium-transporting ATPase subunit alpha-3 Protein SEQUENCE

  • SEQUENCE 1013 AA; 111749 MW; BF28CD9F1E11AF48 CRC64;

    MGDKKDDKDS PKKNKGKERR DLDDLKKEVA MTEHKMSVEE VCRKYNTDCV

    QGLTHSKAQE ILARDGPNAL TPPPTTPEWV KFCRQLFGGF SILLWIGAIL

    CFLAYGIQAG TEDDPSGDNL YLGIVLAAVV IITGCFSYYQ EAKSSKIMES

    FKNMVPQQAL VIREGEKMQV NAEEVVVGDL VEIKGGDRVP ADLRIISAHG

    CKVDNSSLTG ESEPQTRSPD CTHDNPLETR NITFFSTNCV EGTARGVVVA

    TGDRTVMGRI ATLASGLEVG KTPIAIEIEH FIQLITGVAV FLGVSFFILS

    LILGYTWLEA VIFLIGIIVA NVPEGLLATV TVCLTLTAKR MARKNCLVKN

    LEAVETLGST STICSDKTGT LTQNRMTVAH MWFDNQIHEA DTTEDQSGTS

    FDKSSHTWVA LSHIAGLCNR AVFKGGQDNI PVLKRDVAGD ASESALLKCI

    ELSSGSVKLM RERNKKVAEI PFNSTNKYQL SIHETEDPND NRYLLVMKGA

    PERILDRCST ILLQGKEQPL DEEMKEAFQN AYLELGGLGE RVLGFCHYYL

    PEEQFPKGFA FDCDDVNFTT DNLCFVGLMS MIDPPRAAVP DAVGKCRSAG

    IKVIMVTGDH PITAKAIAKG VGIISEGNET VEDIAARLNI PVSQVNPRDA

    KACVIHGTDL KDFTSEQIDE ILQNHTEIVF ARTSPQQKLI IVEGCQRQGA

    IVAVTGDGVN DSPALKKADI GVAMGIAGSD VSKQAADMIL LDDNFASIVT

    GVEEGRLIFD NLKKSIAYTL TSNIPEITPF LLFIMANIPL PLGTITILCI

    DLGTDMVPAI SLAYEAAESD IMKRQPRNPR TDKLVNERLI SMAYGQIGMI

    QALGGFFSYF VILAENGFLP GNLVGIRLNW DDRTVNDLED SYGQQWTYEQ

    RKVVEFTCHT AFFVSIVVVQ WADLIICKTR RNSVFQQGMK NKILIFGLFE

    ETALAAFLSY CPGMDVALRM YPLKPSWWFC AFPYSFLIFV YDEIRKLILR

    RNPGGWVEKE TYY

  • UCSC uc002osg.4; human. [P13637-1];



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