Human CD6 ELISA Kit

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  • Alternative name

    Human T12 ELISA Kit;Human TP120 ELISA Kit;Human CD6 molecule ELISA Kit;Human T-cell differentiation antigen CD6 ELISA Kit;Human CD6 antigen ELISA Kit;

  • Catalog
    E035655
  • species
    Human
  • GeneCD6
  • SpecificityNatural and recombinant human CD6
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman CD6 ELISA Kit allows for the in vitro quantitative determination of CD6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human CD6 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD6 has been precoated onto 96-well plates. Standards(NSO, H18-E398) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD6 amount of sample captured in plate. Background: CD6 (Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What's more, this gene may be associated with susceptibility to multiple sclerosis.
  • Human Soluble CD6 Protein information
  • Uniprot ID CD6_HUMAN
  • Uniprot AC P30203; A4KAD4; A4KAD5; Q9UMF2; Q9Y4K7; Q9Y4K8; Q9Y4K9; Q9Y4L0;
  • UniGene Hs.744366;
  • GeneID 923
  • KEGG hsa:923;
  • Human Soluble CD6 Protein SEQUENCE
  • SEQUENCE 668 AA; 71801 MW; 59E6790E77FFBC42 CRC64;

    MWLFFGITGL LTAALSGHPS PAPPDQLNTS SAESELWEPG ERLPVRLTNG

    SSSCSGTVEV RLEASWEPAC GALWDSRAAE AVCRALGCGG AEAASQLAPP

    TPELPPPPAA GNTSVAANAT LAGAPALLCS GAEWRLCEVV EHACRSDGRR

    ARVTCAENRA LRLVDGGGAC AGRVEMLEHG EWGSVCDDTW DLEDAHVVCR

    QLGCGWAVQA LPGLHFTPGR GPIHRDQVNC SGAEAYLWDC PGLPGQHYCG

    HKEDAGAVCS EHQSWRLTGG ADRCEGQVEV HFRGVWNTVC DSEWYPSEAK

    VLCQSLGCGT AVERPKGLPH SLSGRMYYSC NGEELTLSNC SWRFNNSNLC

    SQSLAARVLC SASRSLHNLS TPEVPASVQT VTIESSVTVK IENKESRELM

    LLIPSIVLGI LLLGSLIFIA FILLRIKGKY ALPVMVNHQH LPTTIPAGSN

    SYQPVPITIP KEVFMLPIQV QAPPPEDSDS GSDSDYEHYD FSAQPPVALT

    TFYNSQRHRV TDEEVQQSRF QMPPLEEGLE ELHASHIPTA NPGHCITDPP

    SLGPQYHPRS NSESSTSSGE DYCNSPKSKL PPWNPQVFSS ERSSFLEQPP

    NLELAGTQPA FSAGPPADDS SSTSSGEWYQ NFQPPPQPPS EEQFGCPGSP

    SPQPDSTDND DYDDISAA

  • UCSC uc001nqq.4; human. [P30203-1];



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