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Alternative name
Sodium/hydrogen exchanger 10 ELISA KIT; Na(+)/H(+) exchanger 10 ELISA KIT; NHE-10 ELISA KIT; Solute carrier family 9 member 10 ELISA KIT; Solute carrier family 9 member C1 ELISA KIT; Sperm-specific Na(+)/H(+) exchanger ELISA KIT; sNHESLC9C1 ELISA KIT; SLC9A10 ELISA KIT; NHE-10 ELISA KIT; sNHE ELISA KIT
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Catalog
E035673
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species
Human
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GenesCD40L
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SpecificityNo cross-reaction with other factors.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.05 ng/ml.
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Intended UseHuman sCD40L ELISA Kit allows for the in vitro quantitative determination of sCD40L , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human sCD40L monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
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