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Alternative name
Sonic hedgehog protein ELISA KIT; HHG-1Cleaved into the following 2 chains:Sonic hedgehog protein N-product ELISA KIT; Sonic hedgehog protein C-productSHH ELISA KIT; SHH ELISA KIT
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Catalog
E036172
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species
Human
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GeneShh-N
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human Shh-N. No significant cross-reactivity or interference between human Shh-N and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human Shh-N is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
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Intended UseHuman Shh-N ELISA Kit allows for the in vitro quantitative determination of Shh-N , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Shh-N has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Shh-N present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Shh-N is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Shh-N bound in the initial step. The color development is stopped and the intensity of the color is measured.
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