Human SMPD1 ELISA Kit

Alternative name
Human acid sphingomyelinase ELISA Kit;Human ASMASE ELISA Kit;Human ASM ELISA Kit;Human NPD ELISA Kit;Human sphingomyelin phosphodiesterase 1 ELISA Kit;Human sphingomyelin phosphodiesterase ELISA Kit;Human sphingomyelin phosphodiesterase 1, acid lysosomal ELISA Kit;
Catalog
E036424
species
Human
GeneSMPD1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.469ng/ml
Intended UseHuman SMPD1 ELISA Kit allows for the in vitro quantitative determination of SMPD1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ASM antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ASM antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ASM amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ASM can be calculated.
Human Sphingomyelin phosphodiesterase Protein information
Uniprot ID ASM_HUMAN
Uniprot AC P17405; A8K8M3; E9PKS3; P17406; Q13811; Q16837; Q16841;
UniGene Hs.498173;
GeneID 6609
KEGG hsa:6609;
Human Sphingomyelin phosphodiesterase Protein SEQUENCE
SEQUENCE 629 AA; 69752 MW; C9888CB8359C42AC CRC64;
MPRYGASLRQ SCPRSGREQG QDGTAGAPGL LWMGLVLALA LALALALSDS
RVLWAPAEAH PLSPQGHPAR LHRIVPRLRD VFGWGNLTCP ICKGLFTAIN
LGLKKEPNVA RVGSVAIKLC NLLKIAPPAV CQSIVHLFED DMVEVWRRSV
LSPSEACGLL LGSTCGHWDI FSSWNISLPT VPKPPPKPPS PPAPGAPVSR
ILFLTDLHWD HDYLEGTDPD CADPLCCRRG SGLPPASRPG AGYWGEYSKC
DLPLRTLESL LSGLGPAGPF DMVYWTGDIP AHDVWHQTRQ DQLRALTTVT
ALVRKFLGPV PVYPAVGNHE STPVNSFPPP FIEGNHSSRW LYEAMAKAWE
PWLPAEALRT LRIGGFYALS PYPGLRLISL NMNFCSRENF WLLINSTDPA
GQLQWLVGEL QAAEDRGDKV HIIGHIPPGH CLKSWSWNYY RIVARYENTL
AAQFFGHTHV DEFEVFYDEE TLSRPLAVAF LAPSATTYIG LNPGYRVYQI
DGNYSGSSHV VLDHETYILN LTQANIPGAI PHWQLLYRAR ETYGLPNTLP
TAWHNLVYRM RGDMQLFQTF WFLYHKGHPP SEPCGTPCRL ATLCAQLSAR
ADSPALCRHL MPDGSLPEAQ SLWPRPLFC
UCSC uc001mcw.4; human. [P17405-1];

Category

Human SMPD1 ELISA Kit

Human acid sphingomyelinase ELISA Kit;Human ASMASE ELISA Kit;Human ASM ELISA Kit;Human NPD ELISA Kit;Human sphingomyelin phosphodiesterase 1 ELISA Kit;Human sphingomyelin phosphodiesterase ELISA Kit;Human sphingomyelin phosphodiesterase 1, acid lysosomal ELISA Kit;