Human S1PR1 ELISA Kit

Alternative name
Human S1P receptor 1 ELISA Kit;Human S1P1 ELISA Kit;Human endothelial differentiation G-protein coupled receptor 1 ELISA Kit;Human sphingosine 1-phosphate receptor Edg-1 ELISA Kit;Human S1P receptor Edg-1 ELISA Kit;Human CHEDG1 ELISA Kit;Human EDG1 ELISA Kit;Human CD363 ELISA Kit;Human D1S3362 ELISA Kit;Human ECGF1 ELISA Kit;Human EDG-1 ELISA Kit;Human sphingosine-1-phosphate receptor 1 ELISA Kit;Human sphingosine 1-phosphate receptor 1 ELISA Kit;Human endothelial differentiation, sphingolipid G-protein-coupled receptor, 1 ELISA Kit;Human sphingosine 1-phosphate receptor EDG1 ELISA Kit;
Catalog
E036436
species
Human
GeneS1PR1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Sphingosine 1 Phosphate Receptor 1 (S1PR1). No significant cross-reactivity or interference between Sphingosine 1 Phosphate Receptor 1 (S1PR1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.058ng/mL.
Intended UseHuman S1PR1 ELISA Kit allows for the in vitro quantitative determination of S1PR1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate Receptor 1 (S1PR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate Receptor 1 (S1PR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate Receptor 1 (S1PR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine 1 Phosphate Receptor 1 (S1PR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Sphingosine 1-phosphate receptor 1 Protein information
Uniprot ID S1PR1_HUMAN
Uniprot AC P21453; D3DT66; Q9BYY4; Q9NYN8;
UniGene Hs.154210;
GeneID 1901
KEGG hsa:1901;
Human Sphingosine 1-phosphate receptor 1 Protein SEQUENCE
SEQUENCE 382 AA; 42811 MW; 0CCE8685A5E1BAD2 CRC64;
MGPTSVPLVK AHRSSVSDYV NYDIIVRHYN YTGKLNISAD KENSIKLTSV
VFILICCFII LENIFVLLTI WKTKKFHRPM YYFIGNLALS DLLAGVAYTA
NLLLSGATTY KLTPAQWFLR EGSMFVALSA SVFSLLAIAI ERYITMLKMK
LHNGSNNFRL FLLISACWVI SLILGGLPIM GWNCISALSS CSTVLPLYHK
HYILFCTTVF TLLLLSIVIL YCRIYSLVRT RSRRLTFRKN ISKASRSSEK
SLALLKTVII VLSVFIACWA PLFILLLLDV GCKVKTCDIL FRAEYFLVLA
VLNSGTNPII YTLTNKEMRR AFIRIMSCCK CPSGDSAGKF KRPIIAGMEF
SRSKSDNSSH PQKDEGDNPE TIMSSGNVNS SS
UCSC uc001dud.3; human.;

Category

Human S1PR1 ELISA Kit